桂西壮族人群PPARγC161-T基因多态性与脑梗死的相关性研究*

[摘要] 目的 探讨桂西地区壮族人群过氧化物酶体增殖物激活受体（PPARγ）C161-T基因多态性与脑梗死的相关性。方法 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测符合入选标准的脑梗死患者114例及120例同期健康人 PPARγ C161→T基因多态性，综合分析基因频率、等位基因频率分布。结果 PPARγC161-T基因基因型分别为CC，CT和TT型。其在脑梗死患者组分布频率分别为76.32%、20.17%、 3.51%，C等位基因频率为86.4%，T等位基因频率为13.6%；正常对照组中为60.0%,34.17%,5.83%，C等位基因频率为77.08%，T等位基因频率为22.92%，两组间各基因频率及等位基因频率差异有显著性，正常对照组T等位基因明显高于脑梗死组（P<0.05）。结论 PPARγ C161→T基因多态性与脑梗死有重要的关联性，T等位基因携带者发生脑梗死的危险性较少。

Relasionship between peroxisome proliferators activated receptor C161-T gene polymorphsm in patients with Brain infarctions of Zhuang nationality Public

[Abstract]: Objective To study whether PPARγC161-T polymorphism was associated whith Brain infarctions of Zhuang nationality Public. Methods The PPARγC161-T gene of 114 Brain infarctions patients and 120 healthy people were amplified with PCR-RFLP. Results There were three kinds of genotype in PPARγC161-T:CC,CT and TT.Among three genotype of the patients,CC was accounted for 76.32%,CT for 20.17%,TT for 3.51%,while the frequency among control subjects were 60.0%,34.17%,5.83%,respectively.The frequency of C alleles was significantly higher in patients than that in normal controls(86.4% and 77.08% ,respectively),And he frequency of T alleles was significantly higher in normal controls than that in patients (22.92% and 13.60% ,respectively). There was a difference statistically in the frequencies of genotype and alleles between two groups（P<0.05）.Conclusion There was an association between PPARγC161-T polymorphism. T alleles carrier to occur brain infarctions were less than C alleles.