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Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay |
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| Authors: | Guoqing Zhang Fangping Cai Ivette Lorenzana de Rivera Zhiyong Zhou Jing Zhang John Nkengasong Feng Gao Chunfu Yang |
| Affiliation: | aInternational Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA;bDepartment of Medicine, Duke University Medical Center, Durham, North Carolina, USA;cMicrobiology Department, National Autonomous University of Honduras, Tegucigalpa, Honduras |
| Abstract: | A multiplex allele-specific (MAS) assay has been developed for the detection of HIV-1 subtype C drug resistance mutations (DRMs). We have optimized the MAS assay to determine subtype B DRMs in dried blood spots (DBS) collected from patients on antiretroviral therapy. The new assay accurately detected DRMs, including low-abundance mutations that were often missed by Sanger sequencing. |
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