苯甲酸雌二醇对去卵巢大鼠海马突触素和钙结合蛋白免疫活性的影响

目的　探讨苯甲酸雌二醇对大鼠海马CA1区、齿状回突触素(synaptophysin, Syn)及钙结合蛋白(calbindinD28k, CaBP, parvalbumin, PV)的调节作用。方法　用大鼠卵巢切除(OVX)动物模型，用免疫组化结合图象分析的方法。结果　OVX组大鼠海马CA1区及齿状回突触素、PV,CaBP积分光密度及面积密度均显著低于对照组，补充苯甲酸雌二醇35 d，这3项指标的积分光密度及面积密度均告恢复，与OVX组相比，呈显著性差异。结论　苯甲酸雌二醇可能通过调节海马、齿状回神经元突触密度，为改善学习记忆功能，缓解AD病人的症状提供结构基础，并通过维持胞内Ca²⁺的恒定达到保护神经元的目的。

EFFECTS OF ESTRADIOL BENZOATE ON SYNAPTOPHYSIN, PARVALBUMIN AND CALBINDIN D28K IMMUNOREACTIVITY IN HIPPOCAMPUS OF OVARIECTOMIZED RATS

AIM　To observe the effects of estradiol benzoate on synaptophysin(Syn), parvalbumin(PV) and calbindin D28k(CaBP) immunoreactivity in hippocampus of ovariectomized(OVX) rats. METHODS　An animal model was developed using ovariectomized(OVX) rats, and immunoreactive technique was used with the help of an image analysis system. RESULTS　OVX in rats caused a decline in both integral optical density and area density of Syn, PV and CaBP immunoreactivity in hippocampus CA1 and dentate gyrus. These effects can be reversed by estradiol benzoate given 200 μg per rat, im, once every three days, for five weeks. CONCLUSION　The increase of expression of the synaptic protein and neuronal calcium binding capacity might be related to both the improvement of memory/learning behavior and the maintenance of neuronal calcium homeostasis.