TRAIL对人卵巢癌SKOV3细胞生长及凋亡的影响

目的 研究TRAIL对卵巢癌SKOV3细胞生长及凋亡的影响。同时了解化疗药物对TRAIL受体表达的影响。方法 用MTT法和流式细胞仪检测不同浓度TRAIL蛋白和顺铂（DDP）联合用药对SKOV3细胞生长的影响；化疗药物处理后用RT—PCR方法检测细胞的TRAIL受体（DR4、DR5）表达的变化。结果 随着TRAIL蛋白浓度的升高，SKOV3细胞抑制率逐渐升高。TRAIL浓度达到10ng／mL及以上浓度时，TRAIL＋DDP联合组的抑制率比TRAIL组明显升高（P〈0．05）。流式细胞仪检测SKOV3正常对照组、TRAIL组和TRAIL＋DDP组细胞凋亡率分别为5．9％、13．4％、39．5％，各实验组与对照组比较差异有统计学意义（P〈0．05），TRAIL蛋白使细胞更多地停留在G0-G1期。RT—PCR检测发现DDP使SKOV3细胞DR5和DR4水平明显升高，分别为对照的1．95倍和1．54倍（P〈0．05），而阿霉素、卡铂、5-FU处理后SKOV3细胞DR4、DR5表达无明显改变。结论 TRAIL蛋白单独使用对SKOV3细胞生长有抑制作用，肿瘤细胞凋亡率升高，TRAIL与DDP联合使SKOV3细胞抑制率明显升高，促凋亡作用更明显。DDP作用后SKOV3细胞DR4、DR5表达成倍增加。

TRAIL Impacts in Growth and Apoptosis of Human Ovarian Carcinoma Cell Line SKOV3

Objective To investigate the effect of TRAIL on growth and apoptosis of ovarian carcinoma cell line SKOV3. Methods The effects of TRAIL alone and/or associating with DDP to treat on SKOV3 cells growth inhibition were measured by MTT assay, and the changes of DR4 and DR5 mRNA were detected by RT-PCR technique. The cell cycle and apoptosis index were analyzed by technique of Flow Cytometry (FCM). Results The soluble TRAIL protein had the inhibiting effect on the growth SKOV3 cells. The DDP increased the ability of TRAIL to SKOV3 cell growth inhibition. The apoptotic indexes (AI) of SKOV3 cells detected by FCM were 5.9%, 13.4% and 39.5% for control group, TRAIL group and TRAIL plus DDP group, respectively. The DDP increased the DR5 expressing in SKOV3 cells by 1.95 folds than control group and by 1.54 times for the DR4 expressing than the control group (P<0.05). Conclusions The soluble TRAIL has the effect on inhibiting the growth of SKOV3 cells. The combination use of TRAIL and DDP increases significantly the growth-inhibiting ratio to SKOV3 cells. The AI of SKOV3 cell is raised after TRAIL intervening in. The DDP can increase the expressions of DR4 and DR5 in SKOV3 cells.