| Stathmin蛋白的研究进展 |
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| 引用本文： | 吴 冰,张立潮,张惠中. Stathmin蛋白的研究进展[J]. 中国肿瘤生物治疗杂志, 2005, 12(1): 71-73 |
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| 作者姓名： | 吴 冰 张立潮 张惠中 |
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| 作者单位： | 复旦大学上海医学院免疫系,教育部分子医学重点实验室,上海基因免疫和疫苗研究中心,上海,200032 |
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| 基金项目： | 教育部科学技术研究项目，国家杰出青年科学基金 |
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| 摘 要： | 目的: 探讨趋化因子受体CXCR4表达水平对人肺癌细胞转移潜能的影响.方法: 采用RT-PCR,FACS检测趋化因子受体CXCR4在肺癌细胞株95C,95D细胞的表达.构建CXCR4正义和反义真核表达质粒,用脂质体法转染至95C和95D细胞内,通过G418筛选出稳定转染株.通过趋化和趋化侵袭实验测定转染前后细胞对基质衍生因子(SDF-1α)的迁移能力,通过明胶酶谱法检测MMP-2活性,通过FACS检测细胞对血管内皮细胞的黏附能力.结果: CXCR4正义和反义真核表达质粒pcDNA3-X4和pcDNA3-ASX4能明显上调或下调肺癌细胞表面CXCR4的表达,上调95C细胞表面CXCR4的表达可以增强其对SDF-1α的趋化反应性、MMP-2活性及其对血管内皮细胞的黏附能力.相反,下调95D细胞表面CXCR4的表达抑制了其对SDF-1α的趋化反应性、MMP-2活性及其对血管内皮细胞的黏附能力.结论: 上调或下调肺癌细胞表面CXCR4表达可以显著增强或抑制其转移潜能,提示CXCR4表达水平与肺癌细胞转移潜能有关.
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| 关 键 词： | 肺癌细胞 转移潜能 |
| 文章编号： | 1007-385X(2005)01-0046-06 |
| 收稿时间： | 2004-07-05 |
| 修稿时间： | 2004-09-27 |
The Effect of the Expression Level of CXCR4 on the Metastatic Potential of Human Lung Cancer Cells |
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| WU Bing,ZHANG Li-chao and ZHANG Hui-zhong. The Effect of the Expression Level of CXCR4 on the Metastatic Potential of Human Lung Cancer Cells[J]. Chinses Journal of Cancer Biotherapy, 2005, 12(1): 71-73 |
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| Authors: | WU Bing ZHANG Li-chao ZHANG Hui-zhong |
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| Affiliation: | Department of Immunology of Shanghai Medical College of Fudan University, Key Laboratory of Molecular Medicine of Ministry of Education, Center for Gene Immunization and Vaccine Research, Shanghai, 200032, China;Department of Immunology of Shanghai Medical College of Fudan University, Key Laboratory of Molecular Medicine of Ministry of Education, Center for Gene Immunization and Vaccine Research, Shanghai, 200032, China;Department of Immunology of Shanghai Medical College of Fudan University, Key Laboratory of Molecular Medicine of Ministry of Education, Center for Gene Immunization and Vaccine Research, Shanghai, 200032, China;Department of Immunology of Shanghai Medical College of Fudan University, Key Laboratory of Molecular Medicine of Ministry of Education, Center for Gene Immunization and Vaccine Research, Shanghai, 200032, China;Department of Immunology of Shanghai Medical College of Fudan University, Key Laboratory of Molecular Medicine of Ministry of Education, Center for Gene Immunization and Vaccine Research, Shanghai, 200032, China |
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| Abstract: | |
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| Keywords: | CXCR4 |
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