The trophic effect of epidermal growth factor on morphological changes and polyamine metabolism in the small intestine of rats |
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Authors: | Tomoyuki Tsujikawa Tadao Bamba Shiro Hosoda |
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Institution: | 1. The Second Department of Internal Medicine, Shiga University of Medical Science, Tsukinowa-cho, Otsu, 520-21, Shiga, Japan
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Abstract: | This study was undertaken to evaluate the effect of epidermal growth factor (EGF) on the morphological changes and polyamine
metabolism in the atrophic small intestinal mucosa of rats caused by feeding elemental diet (ED; Elental?, Ajinomoto, Tokyo) for several weeks. Four-week-old Wistar male rats were given ad libitum ED (1 kcal/ml) for 4 weeks. The
body weight increased to the same extent as the control group fed a pellet diet. However, the small intestine became atrophic:
the mucosal wet weight of the jejunum decreased to 70%, while that of the ileum decreased to 60%. EGF (10 Μg/kg) was subcutaneously
injected into these rats every 8 hours. Ornithine decarboxylase (ODC) activities of the jejunal and ileal mucosa rose within
12 hours of the initial EGF administration. Mucosal DNA specific activities tended to increase. Next, EGF (30 Μg/kg/day) was
intraperitoneally administered with a Mini-osmotic pump for one week. The wet weight, protein and DNA contents of the ileal
mucosa increased significantly compared with those of the saline administered controls, while the crypt cell production rate
(CCPR) also increased. Histologically, increases in both villus height and crypt depth were confirmed. These findings indicate
that EGF causes mucosal proliferation through polyamine metabolism even in the atrophie small intestine of mature rats after
ED administration for 4 weeks. |
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Keywords: | elemental diet epidermal growth factor ornithine decarboxylase |
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