纤溶酶原激活剂抑制物-1基因启动子区-6754G/5G多态性与中国人冠心病的关联研究

目的　研究纤溶酶原激活剂抑制物 - 1(plasminogen activator inhibitor- 1,PAI- 1)基因启动子区 - 6 75 4 G/ 5 G多态性与中国人冠状动脉粥样硬化性心脏病 (coronary heartdisease,CHD,简称冠心病 )发病的关系。方法　应用聚合酶链反应 -限制性片段长度多态性分析 ,对 12 1名健康人和 12 6例冠心病患者(其中急性心肌梗塞 4 7例 ,陈旧性心肌梗塞 39例 ,心绞痛 4 0例 )进行了 PAI- 1基因 4 G/ 5 G多态性分析。结果　 PAI- 1基因 4 G等位基因频率冠心病组 (0 .6 0 )显著高于正常对照组 (0 .4 8) (χ2 =7.6 3,P<0 .0 1) ;4 G/4 G基因型基因频率冠心病组 (0 .397)显著高于正常对照组 (0 .190 ) (χ2 =12 .6 7,P<0 .0 1) ,与 5 G/ 5 G基因型相比 ,对冠心病的比数比 (odds ratio,OR)为 2 .5 4 ,95 %的可信区间 (confidence interval,CI) :1.2 2～ 5 .2 7(P<0 .0 5 ) ,差异有显著性 ,而 4 G/ 5 G基因型对冠心病的 OR为 1.2 8,95 % CI:1.4 5～ 2 .38(P>0 .0 5 ) ,差异无显著性。结论　PAI- 1基因 4 G/ 4 G基因型与中国汉族人冠心病的发病有关联 ,4 G/ 4 G基因型个体易发生冠心病

Association of plasminogen activator inhibitor-1 gene 4G/5G polymorphism and coronary heart disease in Chinese patients

OBJECTIVE: To assess whether the plasminogen activator inhibitor-1 (PAI-1) gene 4G/5G polymorphism is associated with coronary heart disease (CHD) in Chinese patients. METHODS: PAI-1 gene 4G /5G polymorphism was analyzed in normal group (121 individuals) and CHD group (126 cases) by a combination of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). RESULTS: The 4G allele and 4G/4G genotype frequencies of PAI-1 gene (0.60 and 0.397) for CHD patients were higher than those (0.48 and 0.190) for healthy controls(chi-square=7.63 P<0.01; chi-square=12.67, P<0.01).The odds ratios(OR) for CHD in subjects with the 5G/5G (and 4G/5G) genotypes were 2.54 (95% CI 1.22-5.27, P<0.05) and 1.28(95% CI 1.45-2.38, P>0.05), respectively. CONCLUSION: These results suggest that the PAI-1 4G/4G genotype is associated with an increased risk for CHD in Chinese patients. The subjects with the 4G/4G genotype had a higher prevalence of CHD, compared to those with the 5G/5G PAI-1 genotype.