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| 腺病毒介导NDRG2在体外对人食管鳞癌细胞系Eca-109增殖和凋亡的影响 | |
| 引用本文： | 江宁,刘文超,刘学武,王江,刘新平,药立波. 腺病毒介导NDRG2在体外对人食管鳞癌细胞系Eca-109增殖和凋亡的影响[J]. 现代肿瘤医学, 2010, 18(9). DOI: 10.3969/j.issn.1672-4992.2010.09.01 |
| 作者姓名： | 江宁 刘文超 刘学武 王江 刘新平 药立波 |
| 作者单位： | 1. 第四军医大学西京医院肿瘤科,陕西,西安,710032 2. 第四军医大学生物化学与分子生物学教研室,陕西西安,710032 3. 第四军医大学西京脑科医院神经外科,陕西,西安,710032 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的:研究抑癌候选基因NDRG2在体外对人食管鳞癌细胞系Eca-109增殖和凋亡的影响.方法:腺病毒介导NDRG2转染人食管鳞癌细胞系Eca-109,逆转录酶-多聚酶链反应(RT-PCR)和Western blot法分别检测NDRG2基因的mRNA和蛋白表达水平,甲基嚷唑基四唑法(MTT法)绘制细胞生长曲线,流式细胞仪(FCM)分别分析细胞周期和细胞凋亡.结果:腺病毒介导NDRG2转染人食管鳞癌细胞系Eca-109后,NDRG2的mRNA和蛋白表达水平明显上调,细胞增殖受到明显抑制(P＜0.05);流式细胞仪检测显示,与对照组相比,感染后48小时Eca-109细胞G2期细胞明显减少(P＜0.01),S期细胞明显增多(P＜0.01);转染后的Eca-109细胞中凋亡细胞明显增多,感染48h后达16.0%.结论:过表达NDRG2可明显抑制人食管鳞癌细胞Eca-109的增殖并诱导细胞凋亡. |
| 关 键 词： | 食管鳞癌 增殖 凋亡 腺病毒 |
Influence of NDRG2 on proliferation and apoptosis of human esophageal cell line Eca - 109 |
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| JIANG Ning,LIU Wen-chao,LIU Xue-wu,WANG Jiang,LIU Xin-ping,YAO Li-bo. Influence of NDRG2 on proliferation and apoptosis of human esophageal cell line Eca - 109[J]. Journal of Modern Oncology, 2010, 18(9). DOI: 10.3969/j.issn.1672-4992.2010.09.01 | |
| Authors: | JIANG Ning LIU Wen-chao LIU Xue-wu WANG Jiang LIU Xin-ping YAO Li-bo |
| Affiliation: | JIANG Ning1,LIU Wen-chao1,LIU Xue-wu2,WANG Jiang3,LIU Xin-ping2,YAO Li-bo2 1Department of Clinical Oncology,State Key Discipline of Cell Biology,Xijing Hospital,2Department of Biochemistry and Molecular Biology,3Department of Neurosurgery,Xijing Institute of Clinical Neuroscience,Fourth Military Medical University,Xi'an 710032,China. |
| Abstract: | Objective:To investigate the function of candidate tumor suppressor gene NDRG2 on proliferation and apoptosis of human esophageal cancer cell line Eca-109.Methods:Eca-109 cells were transfected with adenovirus vector containing NDRG2 gene. The expression of message RNA and protein of NDRG2 were detected by RT-PCR and Western blot respectively. The cell growth curve was drawn by methyl thiazolyl tetrazolium assay(MTT). Flow cytometry (FCM) was adopted to analyze quantitatively the cell cycle and apoptosis.Re... |
| Keywords: | NDRG2 |
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