A multiplex polymerase chain reaction method for rapid pneumococcal serotype determination in childhood empyema |
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| Authors: | Maataoui Naouale Bidet Philippe Doit Catherine De Lauzanne Agathe Lorrot Mathie Mariani-Kurkdjian Patricia Faye Albert Bingen Edouard |
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| Affiliation: | 1. Service de Microbiologie, Hôpital Robert-Debré (APHP), 75019 Paris, Université Denis Diderot-Paris 7, France;2. Service de Pédiatrie Générale, Hôpital Robert-Debré (APHP), 75019 Paris, Université Denis Diderot-Paris 7, France |
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| Abstract: | Nonvaccine serotypes are increasingly responsible for pneumococcal empyema. We developed a one-step polymerase chain reaction for identifying all serotypes covered by the 13-valent conjugate vaccine. Over 1 year, we identified serotypes 19A (n = 6), 1 (n = 2), 3 (n = 1) and 7F/A (n = 2), both in culture-positive and culture-negative pleural fluid from 11 children with empyema. |
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| Keywords: | Streptococcus pneumoniae Serotype PCR Empyema |
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