Protective effect of dapsone against renal ischemia-reperfusion injury in rat

Abstract

Background: Ischemia/reperfusion can cause injury to tissues and compromise functionality of organs due to inflammatory processes. Significantly, development of these effects in kidney tissue has been a challenging issue that leads to acute renal injury. In this study, anti-inflammatory, anti-oxidative, and protective features of dapsone on kidney ischemia/reperfusion injury were investigated.

Material and methods: Renal ischemia was induced in rats by bilateral renal arteries clamping for 45?min followed by 24?h reperfusion phase. The effects of different doses of dapsone (1, 3, 10?mg/kg) on ischemia/reperfusion injury in kidney tissue were investigated by targeting BUN, Creatinine, LDH, MDA, MPO, IL-1β, TNF-α, and NFκB. In addition histopathological examination was performed by H&E staining method.

Results and discussion: Comparing the findings of this study showed significant reduction in BUN and LDH in 10?mg/kg dapsone received groups, and Cr, MDA, and MPO in 3?mg/kg dapsone received groups. The serum level of TNF-α was significantly decreased with both doses of 3 and 10?mg/kg dapsone. The same results were observed in the serum level of IL-1β and NFκB. Besides, remarkable improvement in histological damages was also observed with dapsone treatment.

Conclusion: These results support the hypothesis that the positive effects of dapsone on the renal ischemia/reperfusion injury are mediated by modulating inflammatory cascades.