Increase of virus yields and releases of Borna disease virus from persistently infected cells |
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Authors: | G Pauli H Ludwig |
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Affiliation: | Institut für Virologie, Freie Universität Berlin, Nordufer 20, 1000 Berlin 65, Germany |
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Abstract: | Borna disease virus grows to low titres in persistently infected cells with an infectious particle to cell ratio of 0.01 to 0.05. Inclusion of n-butyrate in the growth medium enhances infectivity yields up to 1 log. This effect is time and concentration dependent. In hypertonic medium with an excess of NaCl, KCl or Na2SO4 up to 50% of the total infectious virus yield is released from the cells. Released supernatant virus (buoyant density in sucrose rho = 1.22 g/cm3) is more heat stabile than cell-bound virus (rho = 1.18 g/cm3). The access to cell-free (released) virus opens new possibilities for the characterization of this neurotropic agent. |
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Keywords: | Borna disease virus virus release |
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