胶质瘤中促血管生成素2和血管内皮生长因子基因表达的意义

目的 探讨胶质瘤促血管生成素（Ang2）和血管内皮细胞生长因子（VEGF）基因表达的意义。方法 采用逆转录-酶促链反应（RT-PCR）检测52例人脑胶质瘤中Ang2、VEGF基因的表达，同时采用SABC免疫组化方法检测Ang2蛋白的表达。结果 50例脑胶质瘤表达Ang2 mRNA片段．52例脑胶质瘤表达VEGF mRNA片段。Ang2 mRNA与VEGF mRNA表达呈显著性正相关（r=0，816，P〈0．01）；高度恶性胶质瘤Ang2mRNA、VEGF mRNA表达均显著性高于低度恶性者（P〈0．01）。免疫组化染色显示Ang2蛋白主要分布在高度恶性胶质瘤组织的瘤细胞和内皮细胞．而在低度恶性胶质瘤中呈低水平表达。结论 Ang2与VEGF可能在胶质瘤血管生成中起协同性促进作用。

Significance of expressions of angiopoietin 2 and VEGF gene in glioma

Objective To explore expressions of angiopoietin-2(Ang2) and VEGF gene in glioma and its relation with tumor angiogenesis.Methods RT-PCR was used to detect the expressions of Ang2 and VEGF gene in 52 patients with glioma,and SABC immunohistochemical staining was used to detect the Ang2 protein.Results Ang2 mRNA was expressed in 50 human gliomas,and VEGF mRNA in 52 human gliomas.There was a significant positive correlation between them(r=0.816,P<0 01).The Ang2 mRNA and VEGF mRNA expression levels were considerably higher in high-grade malignant glioma than in low-grade glioma(P<0.01).Immunohistochemical staining showed Ang2 protein was distributed mainly in tumor cells and endothelial cells of highly malignant glioma,whereas it was only weakly expressed in low-grade glioma.Conclusion Ang2 and VEGF may have a synergy with each other in glioma angiogenesis.