羟基红花黄色素A缓解脂多糖诱导内皮细胞炎症因子表达升高的研究

目的:研究羟基红花黄色素A(HSYA)抑制脂多糖(LPS)诱导的脐静脉血管内皮细胞(Eahy926细胞)炎症因子表达升高的作用。方法:采用RT-qPCR法测定Toll样受体4(TLR4)、白介素6(IL-6)、白介素1β(IL-1β)和肿瘤坏死因子α(TNFα)mRNA表达水平及ELISA法测定IL-6、IL-1β和TNFα蛋白表达水平。结果:HSYA浓度为5×10-6mol/L、10×10-6mol/L和20×10-6mol/L时可抑制LPS(终浓度为1μg/mL)诱导的Eahy926细胞IL-6、IL-1β和TNFαmRNA(模型组vs.正常组P值均<0.01;HSYA干预中高剂量组vs.模型组P值均<0.01;HSYA干预低剂量组vs.模型组,P值均<0.05)和TNFα蛋白(模型组vs.正常组,P值均<0.01;HSYA干预IL-1β表达的中高剂量组vs.模型组,P值<0.05;HSYA干预TNFα表达的中剂量组vs.模型组,P值<0.05;高剂量组vs.模型组,P值<0.01。HSYA干预IL-6表达的低剂量组vs.模型组,P值<0.05;HSYA干预中高剂量组vs.模型组,P值<0.01)表达的升高,并随HSYA剂量升高可见药效增强。结论:HSYA对LPS诱导的Eahy926细胞TLR4、IL-6、IL-1β、TNFα表达升高有抑制作用。

Study of hydroxysafflow yellow A to attenuate acute lung injury model of endothelial cell inflammatory factor expression induced by LPS

Objective:To study the inhibitory effects of HSYA on the increased expression of the umbilical vein endothelial cells(Eahy926 cells)inflammatory factor induced by lipopolysaccharide(LPS).Methods:RT-q PCR method is used to determine the expression levels of toll like receptor 4(TLR4),interleukin 6(IL-6),1 interleukin(IL-1β) and tumor necrosis factor α(TNF α) mRNA,and ELISA method is used to determine the protein expression levels of IL-6,IL-1β and TNF α.Results: The results showed that after treated with HSYA(5×10-6 mol/L,10×10-6 mol/L and 20×10-6 mol/L),the increased expression levels of TLR4,IL-6,IL-1β and TNF α mRNA(model vs.sham,P<0.01;HSYA 10×10-6 mol/L and 20×10-6 mol/L vs.model,P<0.01;HSYA 5×10-6 mol/L vs.model,P<0.05) and the protein(model vs.sham,P<0.01;HSYA vs.model,P<0.05,or P<0.01) increased expression induced by LPS(1 μg/mL) were inhibited.It was effective.There are significant differences between the relevant groups.Conclusion: It is suggested that the inhibitory effects of HSYA on the increased expression of the endothelial cells(Eahy926 cells),TLR4,IL-6,IL-1β and TNF α is effective.