基因重组人生长激素（r-hGH)对骨质疏松性骨折愈合影响的实验研究

目的　观察基因重组人生长激素 (r- h GH)对骨质疏松性骨折愈合的作用及影响 ,以期为骨质疏松性骨折提供一种有效的治疗方法。方法　选择 8月龄、雌性、SD大鼠 36只 ,随机分为治疗组与对照组 ,每组各 18只。手术方法建立骨质疏松性骨折实验模型后 ,治疗组动物每只每天皮下注射基因重组人生长激素 (r- h GH) 2 .7mg/ kg,连续 10天 ;对照组同法给予等量生理盐水。分别于用药后 2、4、8周检测血浆 IGF- 1浓度 ,并进行骨痂组织骨密度 (BMD)测定、骨痂组织力学强度测试等。结果　血浆 IGF- 浓度 :2周时 ,治疗组血浆 IGF- I浓度值较对照组明显增高 (P<0 .0 0 5) ,4～ 8周时 ,两组血浆 IGF- 浓度值水平相近 (P<0 .5或 P>0 .5) ;骨痂组织 BMD:4周时治疗组较对照组高 (P<0 .2 ) ,8周时却较对照组低 (P<0 .0 0 1) ;力学强度测试 :4～ 8周治疗组骨痂组织各扭转力学强度参数均较对照组高 ,其中最大扭转角在 4周时两组间差异显著 (P<0 .0 5)。结论　外源性基因重组人生长激素 (r- h GH)对实验性骨质疏松性骨折愈合有一定的促进作用。

Effect of recombinant human growth hormone on experimental osteoporotic fracture healing

To observe the effect and influence of recombinant human growth hormome(r hGH)on osteoporotic fracture healing in the rat,and to expect to provide an effective therapy for osteoporotic fracture.Method\ 36 female 8 month old SD rats were randomized into two groups of 18 each,the treatment group and the control group.After the experimental model of osteoporotic fracture by operation was established,the treatment group was given r hGH 2.7 mg/kg body weight/day(1mg-3IU)subcutaneously once a day for cousecutive 10 days,whereas the control group was given equivalent saline as placebo.Plasma IGF I concentration was determined,and bone mineral density(BMD)and biomechanical strength of callus were measured 2,4,8 weeks after the treatment.Results\ Plasma IGF 1 concentration in the treatment group was higher than that in the control group( P <0 005)at 2th week and began to decline at 4th week.At 8th week,there was no significant difference between the two groups( P <0 5 or P >0 5).At 4th week,callus area and BMD in the treatment group were higher than those in the control group,but at 8th week,they were lower and there was significant difference in BMD between the two groups( P <0 001).From 4th to 8 th week,biomechanical testing showed that torsional strength of callus in the treatment group was higher than that in the control group at 4th or 8th week,meanwhile the difference of maximum torsional angle between the two groups was significant( P <0 005).Conclusion\ Exogenous recombinant human growth hormone can stimulate osteoporotic fracture healing in the rat.