义眼台植入对兔眼眶骨细胞的影响

目的 观察眼球摘除和义眼台植入对兔眼眶骨细胞凋亡的影响,初步探讨义眼台植入对眼眶畸形发育防治的作用机制.方法 实验研究.选用同龄、健康、体重相近新西兰幼兔21只,随机分为眼球摘除组、义眼台植入组及正常对照组.1个月龄时对眼球摘除组和义眼台植入组的兔分别行左侧眼球摘除或眼球摘除联合义眼台植入术,正常对照组左侧眼眶作为参照.2个月龄时处死全部兔子,采用光镜、透射电镜和TUNEL法检测其眶骨骨细胞凋亡,并用TUNEL法计量分析3组骨细胞的凋亡情况,采用LSD法对3组骨细胞凋亡率间进行比较.结果 在3组眶骨组织中均可检测到凋亡骨细胞,用TUNEL法观察可见凋亡骨细胞呈散在不规则性分布;TUNEL法计数结果显示眼球摘除组、义眼台植入组及正常对照组骨细胞凋亡率分别为20.56%±2.13%、14.29%±3.60%、12.57%±2.40%,眼球摘除组骨细胞凋亡率与义眼台植入组及正常对照组骨细胞凋亡率之间差异有统计学意义(P<0.01),义眼台植入组和正常对照组骨细胞凋亡率之间差异无统计学意义(P>0.05).结论 眶骨细胞凋亡参与了义眼台植入防治眼球摘除后眼眶畸形发育的过程.(中华眼科杂志,2008,44:700-704)

Effects of placement of orbital implant an orbital osteocytes

Objective To investigate the influence of enucleation with or without orbital implant on the occurrence of apoptosis of orbital osteocytes and to explore the mechanism of orbital implant in the prevention and treatment of orbital malformations. Methods It was an experimental research. Twenty-one age and weight-matched New Zealand rabbits were divided into three groups: enucleation group, enucleaion with implant group and the control group. At one-month-old, the left eyes of the rabbits were enucleated in the enucleation group; an orbital implant was inserted after enucleation in the implant group. The left orbits of rabbits in the control group were served as the controls. At two-month-old, all rabbits were sacrificed,apoptosis of osteocytes of the zygomatic bones was observed by photomicroscopy, electron microscopy and TUNEL staining technology. The ratios of apoptosis osteocytes were calculated and analyzed in these three groups. Results Classical apoptosis of asteocytes was found with photomicroscnpy and electron microscopy. The distribution of apoptosis of osteocytes was irregular in bone sections. The ratio of apoptosis cells in the enueleation group was significantly different from that in the other two groups (P<0.01). There was no significant difference in ratios of apoptosis cells between the implant group and the controls (P>0.05). Conclusions Apoptasis of osteocytes participitates in the normal development of bony orbit. The results of this study indicate that apoptosis of asteocytes plays a role in the development of orbital malformation after enucleation and orbital implant can prevent the orbital malformation after enucleation. (Chin J Ophthalmol, 2008,44:700-704)