| 靶向ATP6L的RNA干扰抑制乳腺癌细胞的体外侵袭能力 |
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| 引用本文： | 游海燕,金洁,唐宁,邓云,舒惠群,沈秋瑾,覃文新. 靶向ATP6L的RNA干扰抑制乳腺癌细胞的体外侵袭能力[J]. 江苏大学学报(医学版), 2010, 20(2): 126-130,135 |
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| 作者姓名： | 游海燕 金洁 唐宁 邓云 舒惠群 沈秋瑾 覃文新 |
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| 作者单位： | 1. 上海交通大学肿瘤研究所癌基因及相关基因国家重点实验室,上海,200032
2. 江苏大学基础医学与医学技术学院,江苏,镇江,212013 |
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| 基金项目： | 国家自然科学基金资助项目，国家重点基础研究发展计划973课题 |
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| 摘 要： | 目的:通过靶向ATP6L的RNA干扰抑制质子泵的功能,检测MCF-7/ADR乳腺癌细胞的体外侵袭情况.方法:采用有效ATP6L干扰片段及干扰对照分别转染MCF-7/ADR细胞,通过荧光分光光度计检测BCECF与细胞分泌上清孵育后在不同激发光激发后发射的荧光量;用实时荧光定量PCR检测细胞中基质金属蛋白酶2(MMP-2)的表达情况;用明胶酶谱方法检测细胞分泌上清中的MMP-2活性;用细胞侵袭实验(Transwell) 检测细胞体外侵袭能力.结果:在MCF-7/ADR细胞中,下调ATP6L基因的表达可以使细胞的泌H+能力减弱;细胞中MMP-2表达水平无变化;细胞分泌上清中的MMP-2活性减弱;细胞的体外侵袭能力下降.结论:靶向ATP6L的RNA干扰可抑制质子泵的功能,从而抑制MCF-7/ADR乳腺癌细胞的体外侵袭能力.
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| 关 键 词： | RNA干扰 侵袭 肿瘤 |
Small interfering RNA targeting the subunit ATP6L of proton pump V-ATPase suppresses the invasion of breast cancer cells in vitro |
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| YOU Hai-yan,JIN Jie,TANG Ning,DENG Yun,SHU Hui-qun,SHEN Qiu-jin,QIN Wen-xin. Small interfering RNA targeting the subunit ATP6L of proton pump V-ATPase suppresses the invasion of breast cancer cells in vitro[J]. Journal of Jiangsu University Medicine Edition, 2010, 20(2): 126-130,135 |
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| Authors: | YOU Hai-yan JIN Jie TANG Ning DENG Yun SHU Hui-qun SHEN Qiu-jin QIN Wen-xin |
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| Affiliation: | YOU Hai-yan1,JIN Jie2,TANG Ning1,DENG Yun1,SHU Hui-qun1,SHEN Qiu-jin1,QIN Wen-xin1(1.State Key Laboratory for Oncogenes , Related Genes,Shanghai Jiao Tong University Cancer Institute,Shanghai 200032,2.School of Medical Science , Laboratory Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
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| Abstract: | Objective:To explore whether the invasion of MCF-7/ADR cells could be affected by in vitro inhibition of V-ATPase activity.Methods:The expression of ATP6L was knocked down by specific siRNA in breast cancer cell line,MCF-7/ADR.Then the capability of proton extrusion was analyzed by detecting the extracellular pH of the supernatant using pH-sensitive fluorescent dye BCECF on a luminescence spectrometer.The expression of MMP-2 and activity of gelatinase were determinated by real-time fluorogentic quantitative... |
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| Keywords: | ATP6L |
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