清肺止哮汤对哮喘小鼠气道炎症的抑制及Th1/Th2平衡的影响

目的：探讨中药复方清肺止哮汤对哮喘小鼠气道炎症和外周血Th1/Th2平衡的影响。方法：用卵白蛋白（OVA）致敏、激发建立哮喘小鼠模型。末次抗原激发后48h收集血、支气管肺泡灌洗液（BALF）和骨髓标本,计数细胞总数和嗜酸性细胞（Eos）,检测外周血Th1/Th2反应,以及肺组织学检查。结果：清肺止哮汤可明显减少BALF中Eos数,能使肺组织中细支气管、血管周围的Eos浸润和黏液的过度分泌得到控制,但和布地奈德（BUD）组相比仍有显著性差异（P〈0.01）。经中药干预后外周血总的CD3^＋T细胞表达IL-4的细胞群/表达IFN-γ的细胞群与OVA组相比有显著性差异（P〈0.05）,其中Tc2/Tc1,Th2/Th1呈明显下降趋势。结论：清肺止哮汤能部分抑制哮喘小鼠气道炎症,并使外周血CD3^＋T表达IFN-γ的细胞群增加,对于哮喘时Th1/Th2失衡具有一定的纠偏作用。

The Chinese Herbal Medicine Formula QingFei ZhiXiao Tang Suppresses Allergic Airway Inflammation and Modulates Th1/Th2 Balance in a Murine Model of Asthma

Objective:To investigate the effects of The Chinese herbal medicine formula QingFei ZhiXiao Tang,derived from traditional Chinese Medicine(TCMs)used to treat allergic asthma,on a well-characterized murine model of asthma.Methods:C57BL/6 mice were sensitized intraperitoneally and challenged by aerosolizing ovalbumin (OVA) to establish an asthmatic model. QingFei ZhiXiao Tang,according to the corresponding dose was administered intragastrically after the first sensitized. 48h after the last challenge,peripheral blood, bronchoalveolar lavage fluid (BALF) and bone marrow cell suspension were collected and counted. The lungs were fixed and paraffin sections were stained with hematoxylin and PAS.Th1/Th2 balance in peripheral blood was detected by flow cytometry.Results:An optimal dose of QingFei ZhiXiao Tang 2.5g/mL for 26 days was used in this study, which was the dose to inhibit inflammation of the airways. QingFei ZhiXiao Tang markedly reduced the number of eosinophils in bronchoalveolar lavage fluids (BALF) compared with those in OVA group (P<0.01), but there was statistically significant difference between QingFei ZhiXiao Tang and Budesonide treatment (P<0.01). Lung histologic examination also demonstrated that QingFei ZhiXiao Tang reduced allergic airway inflammation and mucus hypersecretion. The effects of QingFei ZhiXiao Tang were comparable to those of budesonide. By intracellular cytokine staining and flow cytometric analysis, we found that Th1/Th2 pattern was shifted to Th2 in OVA-challenged mice. QingFei ZhiXiao Tang reversed that pattern to Th1 toward.Conclusion:The Chinese herbal medicine formula QingFei ZhiXiao Tang inhibits allergen-induced airway inflammation and shows immunoregulatory effects of Th1/Th2 responses which QingFei ZhiXiao Tang up-regulates IFN-gamma-positive cell subsets in CD3+ in a well-characterized murine model of asthma.