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| 蛛网膜下腔出血Synapsin Ⅰ及其磷酸化水平动态变化的研究 | |
| 引用本文： | 袁慧,焉传祝,毛蕾蕾,杨明峰,王锐. 蛛网膜下腔出血Synapsin Ⅰ及其磷酸化水平动态变化的研究[J]. 中国矫形外科杂志, 2011, 19(19): 1634-1637 |
| 作者姓名： | 袁慧 焉传祝 毛蕾蕾 杨明峰 王锐 |
| 作者单位： | 1. 山东大学齐鲁医院神经内科,济南250021;泰山医学院附属医院神经内科,山东省高校脑微循环重点实验室,泰安271000 2. 山东大学齐鲁医院神经内科,济南,250021 3. 泰山医学院附属医院神经内科,山东省高校脑微循环重点实验室,泰安271000 |
| 基金项目： | 国家自然科学基金资助项目(30800358) |
| 摘 要： | [目的]研究蛛网膜下腔出血(SAH)大鼠突触蛋白SynapsinⅠ及其磷酸化水平的动态变化,探讨SynapsinⅠ及其磷酸化在SAH发生发展过程中的作用.[方法]将Wistar大鼠随机分入正常对照组、假手术组和SAH1h,1、3、5、14d组.用枕大池二次注血法制作SAH模型,用激光多普勒血流计检测皮层局部脑血流量(rCBF)动态变化；采用免疫荧光化学及Westernblot法检测皮层SynapsinⅠ及其磷酸化蛋白表达.[结果]SAH后rCBF动态下降,1h、3d组下降明显(P＜0.01).SAH各亚组顶叶皮层SynapsinⅠ表达均下降,1、3、5d组差异有显著性(P＜0.01),磷酸化SynapsinⅠ表达持续低水平,3d组最明显(P＜0.01).[结论]SAH后存在突触受损及突触传递障碍. |
| 关 键 词： | 蛛网膜下腔出血 脑血管痉挛 synapsin Ⅰ 磷酸化synapsin Ⅰ 大鼠 |
Phosphorylation of synapsin I following subarachnoid hemorrhage in rats |
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| YUAN Hui,YAN Chuan-zhu,MAO Lei-lei,et al.. Phosphorylation of synapsin I following subarachnoid hemorrhage in rats[J]. Orthopedic Journal of China, 2011, 19(19): 1634-1637 | |
| Authors: | YUAN Hui YAN Chuan-zhu MAO Lei-lei et al. |
| Affiliation: | YUAN Hui,YAN Chuan-zhu,MAO Lei-lei,et al.Department of Neurology,Shandong Univeristy Qilu Hospital,Jinan 250021,China |
| Abstract: | [Objective]This study aimed to investigate the possible role of phosphorylation of synapsin I in the process of cerebral injury following subarachnoid hemorrhage(SAH). [Methods]Wistar rats were divided into non-SAH,sham-operated group,SAH60min,SAH1d,SAH3d,SAH5d and SAH 14d groups.double injections of blood into cistern magna of rats were performed to induce SAH.Synapsin I protein and phosphosynapsin I protein were measured from cerebral cortex by immunohistochemistry and Western blot. [Results]Synapsin I an... |
| Keywords: | subarachnoid hemorrhage cerebral vasospasm synapsin I phosphosynapsin I rat |
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