| HepaCAM基因转染对膀胱癌BIU-87细胞株生长及血管内皮生长因子表达的影响 |
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| 引用本文： | 杨淑哲,罗春丽,吴小候,潘翠翠,蔡晓钟. HepaCAM基因转染对膀胱癌BIU-87细胞株生长及血管内皮生长因子表达的影响[J]. 第三军医大学学报, 2010, 32(5): 490-493 |
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| 作者姓名： | 杨淑哲 罗春丽 吴小候 潘翠翠 蔡晓钟 |
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| 作者单位： | 重庆医科大学医学检验系教育部重点实验室,重庆,400016;重庆医科大学附属第一医院泌尿外科,重庆,400016 |
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| 摘 要： | 目的探讨外源性肝细胞黏附分子(hepatocyte cell adhesion molecule,hepaCAM)转染对人膀胱癌BIU-87细胞株生长及血管内皮生长因子(vascular endothelial growth factors,VEGF)表达的影响。方法使用脂质体2000转染法将携带野生型hepaCAM基因的质粒(pEGFPN2-hepaCAM)及空载质粒(pEGFPN2)转染人膀胱癌BIU-87细胞,建立稳定过表达hepaCAM基因的BIU-87细胞株。以转染空载质粒及未转染的BIU-87细胞株作为对照,采用MTT法检测各组细胞生长能力,逆转录聚合酶链反应(RT-PCR)检测hepaCAM、VEGF mRNA水平的变化,Western blot检测VEGF蛋白水平的变化。结果MTT实验示:hepaCAM基因转染(实验组)BIU-87细胞生长与空载体组和未处理组细胞比较明显受到抑制(P<0.05);hepaCAM基因在膀胱癌BIU-87细胞不表达,转染后hepaCAM mRNA明显增强(P<0.05);实验组VEGF mRNA表达(0.527±0.031)与未处理组(0.803±0.042)、空...
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| 关 键 词： | 膀胱肿瘤 hepaCAM 血管内皮生长因子类 基因 肿瘤抑制 |
Transfection of wild type hepaCAM gene inhibits cell growth and VEGF expression in BIU-87 cells |
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| Yang Shuzhe,Luo Chunli,Wu Xiaohou,Pan Cuicui,Cai Xiaozhong. Transfection of wild type hepaCAM gene inhibits cell growth and VEGF expression in BIU-87 cells[J]. Acta Academiae Medicinae Militaris Tertiae, 2010, 32(5): 490-493 |
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| Authors: | Yang Shuzhe Luo Chunli Wu Xiaohou Pan Cuicui Cai Xiaozhong |
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| Affiliation: | 1Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education;Faculty of Laboratory Medicine;2 Department of Urinary Surgery;First Affiliated Hospital;Chongqing Medical Uni-versity;Chongqing;400016;China |
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| Abstract: | Objective To investigate the effects of transfection of wild type hepatocyte cell adhesion molecule(hepaCAM) gene on the growth and metastasis of bladder cancer BIU-87 cells.Methods Plasmids pEGFPN2-hepaCAM and pEGFPN2 were respectively transfected into BIU-87 cells with Lipofectamine2000 to establish BIU-87 cells with stable over-expression of hepaCAM.MTT assay was used to observe the proliferation of hepaCAM transfection cells,pEGFPN2 transfection cells and untransfected cells.The mRNA expressions of hepa... |
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| Keywords: | urinary bladder neoplasms hepatocyte cell adhesion molecule vascular endothelial growth factors genes tumor suppressor |
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