HBV-DNA含量与HBV-M的关系探讨

目的：探讨乙型肝炎病毒DNA含量与乙型肝炎病毒血清标志物（HBV-M）之间的关系，为临床诊断及治疗提供有价值的依据。方法：用ELISA检HBV-M，荧光定量聚合酶链反应（FQ-PCR）检HBV-DNA的含量。结果：HBsAg、HBeAg、抗HBc阳性组；HBsAg、HBeAg阳性组；HBsAg，抗HBC阳性组；HBsAg、抗HBe、抗HBc阳性组；抗HBs、抗HBe、抗HBc阳性组及抗HBc阳性组中，HBV-DNA阳性率分别为：96．4％、98．0％、73．8％、51．2％、16．4％及7．4％。结论：PCR检测HBV-DNA含量与HBV—M同时测定，更有利于乙型肝炎的正确诊断，疗效判断及预后观察。

Relationship between the serum HBV marker and the content of HBV-DNA

Objective:To investigate the relationship between the serum HBV marker(HBV-M) and the content of HBV-DNA,in order to provide the valuable evidence for clinical diagnosis and treatment.Methods:The serum HBV marker and the content of HBV-DNA were detected with ELISA and fluorescent quantitative PCR method respectively.Results:In the six common types of serum marker(1.HBsAg,HBeAg and HBcAb were positive;2.HBsAg and HBeAg were positive;3.HBsAg and HBcAg were positive;4.HBsAg,HBeAb and HBcAb were positive;5.HBsAb,HBeAb and HBcAb were positive,6.only the HBcAb was positive),the positive rate of HBV-DNA was 96.4%,73.8%,16.4%,98.0%,51.2%,7.4% respectively.Conclusion:In order to make an accurate diagnosis,observe the curative effect and judge the prognosis,the tests of the serum HBV marker and the content of HBV-DNA should be useful at the same time.