双花解毒合剂质量控制研究

目的：研究双花解毒合剂的质量控制方法，提高其质量标准。方法：采用薄层色谱法对制剂中的金银花、黄芩进行定性鉴别；采用高效液相色谱法对制剂中的有效成分黄芩苷进行含量测定。结果：薄层色谱鉴别的色谱斑点清晰、阴性对照无干扰；黄芩苷质量0.0282～1.128μg有良好的线性范围，r=0.9996，平均加样回收率（n=5）为99.72%，RSD=0.94%。结论：本文建立方法准确可靠、灵敏度高、专属性强，可有效控制双花解毒合剂的质量。

Quality control of double flower detoxification mixture

Objective：To study the quality control of the double flowers detoxifying mixture.Methods：Lonicerae Japonica Flos and Scutellariae Radix in the mixture were qualitatively identified by TLC. The effective constituent baicalin in the preparation was determined by HPLC as the index. Results：The developed TLC spots were clear and without interference of negative sample. The linear range of baicalin was 0.0282~1.128μg, r=0.9996. The average recovery rate was 99.72%, and RSD was 0.94%. Conclusion：This method is proved to be reliable, accurate, specific and reproducible. It can be used for the quality control of the double flower detoxifying mixture.