MMP-9基因启动子C1562T多态性与老年冠心病易感性的相关性研究

目的探讨基质金属蛋白酶9(MMP-9)基因启动子C1562T多态性与老年冠心病(CHD)易感性的相关性。方法纳入2009年3月~2012年12月北京航天总医院年龄≥60岁的CHD患者168例作为CHD组,纳入同期健康体检者208例作为对照组。取外周血标本提取DNA,采用聚合酶链式反应-限制性内切酶片段长度多态性(PCR-RFLP)方法检测MMP-9基因启动子C1562T基因型,比较两组间MMP-9基因多态性频率分布的差异。结果 MMP-9基因启动子区C1562T多态性基因型和等位基因频率分布在CHD组和对照组之间差异有统计学意义(P0.05)。CHD组1562T等位基因频率明显高于对照组(20.8%vs.13.0%,P=0.004),携带T等位基因个体患冠心病的风险是C等位基因的3.97倍(OR=3.97)。不同临床类型的CHD患者在基因型及等位基因分布方面的差异无统计学意义(P0.05)。结论 MMP-9基因C1562T多态性与老年CHD的发生有关联,T等位基因可能是冠心病发病的遗传易感基因。

Correlation between C1562T genetic polymorphism of MMP-9 and susceptivity of senile coronary heart disease

Objective To investigate the correlation between C1562T genetic polymorphism of matrix metalloproteinase-9 （MMP-9） and susceptivity of senile coronary heart disease （CHD）. Methods The patients （aged≥60, n=168） were chosen as CHD group from the Beijing Aerospace General Hospital from Mar. 2009 to Dec. 2012, and 208 persons with normal physical examinations were chosen as control group. The samples of peripheral blood were collected for extracting DNA. The genotypes of C1562T of MMP-9 were detected by using polymerase chain reaction-restricted fragment length polymorphisms （PCR-RFLP）, and difference in frequency distribution of C1562T genetic polymorphism was compared between two groups. Results The differences in genotypes of C1562T genetic polymorphism and allele frequency distribution had statistical significance between two groups （P〈0.05）. In CHD group, the frequency of allele 1562T was significantly higher than that in control group （20.8%vs. 13.0%, P=0.004）. The risk of suffering from CHD in the patients carried allele T was 3.97 times higher than that in those carried allele C （OR=3.97）. The distributions of genotypes and allele had no statistical difference （P〈0.05） among different clinical types of CHD （stable angina, unstable angina and myocardial infarction）. Conclusion C1562T genetic polymorphism of MMP-9 is correlated to senile CHD, and allele T may be a susceptive heredity gene.