| Effects of Co-grafts Mesenchymal Stem Cells and Nerve Growth Factor Suspension in the Repair of Spinal Cord Injury |
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| 作者姓名： | 方煌 王俊芳 陈安民 |
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| 作者单位： | Effects of Co-grafts Mesenchymal Stem Cells and Nerve Growth Factor Suspension in the Repair of Spinal Cord Injury，Effects of Co-grafts Mesenchymal Stem Cells and Nerve Growth Factor Suspension in the Repair of Spinal Cord Injury，Effects of Co-grafts Mesenchymal Stem Cells and Nerve Growth Factor Suspension in the Repair of Spinal Cord Injury |
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| 摘 要： | Endogenous repair ability of mammalsisli mit-ed after the damage of central nervous system.It isdifficult to produce the new neurons for the dam-aged adult central nervous system,and neither canthe damaged systemstart the functional axonal re-generation. At present ,a good many methods arebelieve to be able toi mprove the damagedlocal mi-croenvironment , such as cell transplantation andprovision of exogenous neurotrophic factors ,whichare ai med at promoting the regeneration of thedamaged spin…
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| 收稿时间： | 28 December 2005 |
Effects of Co-grafts Mesenchymal Stem Cells and Nerve Growth Factor Suspension in the Repair of Spinal Cord Injury |
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| FANG Huang,WANG Junfang,CHEN Anmin.Effects of Co-grafts Mesenchymal Stem Cells and Nerve Growth Factor Suspension in the Repair of Spinal Cord Injury[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(2):206-210. |
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| Authors: | FANG Huang WANG Junfang CHEN Anmin |
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| Institution: | (1) Institute of Neurobiology, Center of Excellence, SAS, ?olt?sovej 4, Ko?ice, 04001, Slovakia;(2) Associated Tissue Bank, Faculty of Medicine and University Hospital, P.J. ?af?rik University, Trieda SNP 1, Ko?ice, 04011, Slovakia;(3) UVM, Komensk?ho 72, Ko?ice, Slovakia;(4) Institute of Neurobiology, Center of Excellence, SAS, ?olt?sovej 4, Ko?ice, 04001, Slovakia |
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| Abstract: | Summary To investigate effect of the transplantation of mesenchymal stem cells (MSCs) in combination with nerve growth factor (NGF)
on the repair of spinal cord injury (SCI) in adult rats, spinal cord of adult rats (n=32) was injured by using the modified Allen's method. One week after the injury, the injured cords were injected with Dubecco-modified
Eagles medium (DMEM, Group I), MSCs (Group II), NGF (Group III), and MSCs plus NGF (Group IV). One month and two months after
the injury, rats were sacrificed and their injured cord tissues were sectioned for the identification of the transplanted
cells. The axonal regeneration and the differentiation of MSCs were examined by immunocytochemical staining. At the same time,
rats were subjected to behavioral tests by using the open-field BBB scoring system. Immunocytochemical staining showed that
axonal regeneration and the transplanted cells partially expressed neuron-specific nuclear protein (NeuN) and glial fibrillary
acidic protein (GFAP). At the same time, significant improvement in BBB locomotor rating scale (P<0.05) were observed in the treatment group. More importantly, further functional improvement were noted in the combined treatment
group. MSCs could differentiate into neurons and astrocytes. MSCs and NGF can promote axonal regeneration and improve functional
recovery. There might exist a synergistic effect between MSCs and NGF.
Fang Huang, male, born in 1962, Associate Professor |
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| Keywords: | spinal cord injury bone marrow mesenchymal stem cells nerve growth factor transplantation |
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