| 新生大鼠缺血缺氧性脑病脑神经物质的变化 |
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| 引用本文： | 黎萍,陈晓晴,胡菲菲.新生大鼠缺血缺氧性脑病脑神经物质的变化[J].临床医学工程,2014(3):281-282,285. |
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| 作者姓名： | 黎萍 陈晓晴 胡菲菲 |
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| 作者单位： | [1] 广州医科大学第三附属医院儿科,广东广州510150 [2] 广州中医药大学第一附属医院新生儿科,广东广州510405 |
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| 基金项目： | 广东省医学科学研究基金项目“新生大鼠缺血缺氧过程脑内神经化学物质变化的研究”（项目编号：A2007275） |
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| 摘 要： | 目的运用离体高分辨质子磁共振波谱学方法对新生大鼠缺血缺氧性脑病神经化学物质的变化进行检测,探讨其在缺血缺氧脑损伤的病理生理过程中的作用。方法健康Sprague-Dawley新生大鼠40只,采用完全随机设计将大鼠分为实验组(缺血缺氧组,n=20)和对照组(n=20),运用离体高分辨质子磁共振波谱分析观察新生大鼠缺血缺氧脑损伤后脑皮质内的神经化学物质乳酸(Lac)、谷氨酸(Glu)、天门冬氨酸(Asp)浓度的变化。结果与对照组相比,缺血缺氧组大鼠脑皮质Glu、Asp和Lac总浓度显著升高(P<0.05)。结论缺血缺氧促进脑组织Glu、Asp和Lac的释放,采用离体高分辨质子磁共振波谱学方法有助于研究缺血缺氧脑病的神经化学机制以及组织细胞代谢变化。
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| 关 键 词： | Sprague-Dawley大鼠 缺血缺氧性脑病 离体高分辨质子磁共振波谱学 神经化学物质 |
Anoxaemia Induces the Neurochemical Changes in the Brain Tissues of Rats |
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| Institution: | (1 Department of Pediatrics, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Chino,＂ 2 Department of Neonatology, the First Affiliated Hospital of Guangzhou Traditional Chinese Medicine University, Guangzhou 510405, China) |
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| Abstract: | Objective Ex vivo high resolution H nuclear magnetic resonance spectroscopy （~H-NMRS） was used to measure the alterations of multiple neurochemicals in new-born rat＇s brain tissues suffered from hypoxic ischemic encephalopathy. Methods Forty 7 -day-old Sprague-Dawley rats were randomly divided into experimental group and control group equally. Lactate, glutamate and aspartic acid was measured using ＇H-NMRS. Results The concentration of lactate, glutamate and aspartic acid significantly increased after hypoxic ischemic encephalopathy （P 〈0.05）. Conclusions Hypoxic ischemic encephalopathy improves the release of acetate, glutamate and aspartic acid, JH-NMRS is an effective method to study the mechanism and changes of the neurochemicals in hypoxic ischemic encephalopathy. |
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| Keywords: | Sprague-Dawley rats Hypoxic ischemic encephalopathy Ex vivo high resolution H nuclear magnetic resonancespectroscopy Neurochemicals |
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