| FasL蛋白及其相互作用蛋白对直肠癌细胞耐药性影响的研究 |
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| 引用本文： | 左富义,李世拥,安萍,于波,蔡惠芸. FasL蛋白及其相互作用蛋白对直肠癌细胞耐药性影响的研究[J]. 华北国防医药, 2008, 20(3): 14-16 |
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| 作者姓名： | 左富义 李世拥 安萍 于波 蔡惠芸 |
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| 作者单位： | [1]北京军区总医院全军普通外科中心,北京100700 [2]北京军区总医院中心实验科,北京100700 |
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| 基金项目： | 国家自然科学基金 , 全军科技攻关课题资助项目 |
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| 摘 要： | 目的:探讨FasL蛋白及其相互作用蛋白在直肠癌细胞耐药中的作用机制.方法:利用酵母双杂交系统在相互作用蛋白研究中的特性,筛选与FasL蛋白相互作用较强的蛋白质,通过细胞学实验(MTT法)研究FasL蛋白及其相互作用蛋白对直肠癌细胞耐药性的影响.结果:经过筛选发现,FasL蛋白与anchor attachment蛋白、金属硫蛋白1K具有较强的相互作用.经细胞耐药学实验证实.FasL蛋白、anchor attachment蛋白与金属硫蛋白1K组直肠癌HB-8348细胞的耐药性明显高于其他组细胞,差异具有非常显著性统计学意义(P<0.01),FasL蛋白组以及FasL蛋白和金属硫蛋白1K组直肠癌HP-8348细胞的耐药性均高于空白对照组,差异具有显著性统计学意义(P<0.05).结论:FasL蛋白与anchor attachment蛋白1K结合后,增强了金属硫蛋白1K在结直肠癌细胞中的耐药能力,可能促进了结直肠癌的复发与转移.
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| 关 键 词： | 结直肠肿瘤 复发 肿瘤转移 酵母双杂交 FasL蛋白 细胞耐药性 |
FasL and Its Interacted Proteins in the Drug-resistance of Colorectal Carcinoma |
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| ZUO Fu-yi a,LI Shi-yong a,AN Pingb,YU Bo a,CAI Hui-yun a. FasL and Its Interacted Proteins in the Drug-resistance of Colorectal Carcinoma[J]. Medical Journal of Beijing Military Region, 2008, 20(3): 14-16 |
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| Authors: | ZUO Fu-yi a LI Shi-yong a AN Pingb YU Bo a CAI Hui-yun a |
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| Affiliation: | ZUO Fu-yi a,LI Shi-yong a,AN Pingb,YU Bo a,CAI Hui-yun a(a.Center of General Surgery of PLA,b.Laboratory Center,General Hospital of Beijing Military Area,Beijing 100700,China) |
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| Abstract: | Objective:To screen the proteins which interact with FasL and investigate the mechanism of drug-resistance colorectal carcinoma.Methods:The fetal liver cDNA library was screened using the yeast double-hybrid system and a series of specific proteins that interacted with FasL protein were obtained.We used cytologic method and analyzed interacted proteins to see how they influenced the drug-resistance of colorectal carcinoma.Results:It was found through screening that several proteins interacted strongly with ... |
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| Keywords: | Colorectal neoplasm Recurrence Neoplasm metastasis Yeast double-hybrid Protein FasL Cell drug resistance |
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