E-cad和CD44v6在口腔鳞癌中的表达及意义

目的 探讨黏附分子E-cad和CD44v6在口腔鳞癌中的表达及其与口腔颌面部鳞癌病理分级和侵袭转移的关系.方法 采用免疫组织化学抗生蛋白链菌素-生物素标记染色技术,对54例口腔颌面部鳞癌组织和15例口腔颌面部正常组织进行E-cad和CD44v6表达的检测.结果 口腔鳞癌组织E-cad阳性表达率(50.00%)低于口腔正常组织的阳性表达率(86.67%),其差异有统计学意义(P＜0.05);E-cad和CD44v6的阳性表达率随鳞癌分化程度的降低而降低(P＜0.05);有淋巴结转移的鳞癌原发灶与无淋巴结转移的原发灶比较,前者E-cad阳性表达率(21.43%)低于后者(60.00%),其差异有统计学意义(P＜0.05).E-cad的表达与CD44v6的表达具有显著相关性(P＜0.01).结论 E-cad的低表达与口腔颌面部鳞癌病理分级和转移关系密切.CD44v6在低分化鳞癌组织中低表达.E-cad与CD44v6的联合检测可作为口腔鳞癌发生发展和侵袭转移的参考指标.

Significance and expression of E-cad and CD44v6 in oral and maxillofacial squamous cell carcinoma

Objective To investigate the role of the adhesion molecule E-cad and CD44v6, and the relationship betweenthe expression of E-cad and CD44v6 and the histopathological grade and lymph nodes metastasis in oral andmaxillofacial squamous cell carcinoma(SCC). Methods Immunohistochemical method(LSAB) was applied to examine the expression of E-cad and CD44v6 in 54 oral and maxillofacial SCC and 15 non-cancerous tissues. Results E-cad expression was positive in 50.00% of SCC versus in 86.67% of non-cancerous tissues. The positive rate of E-cad in SCC was lower than that in non-cancerous tissues(P<0.05). Unusual expression of E-cad and CD44v6 was significantly correlated with histopathological grade(P<0.05). The oral and maxillofacial SCC with low positive E-cad expression(21.43%)metastasized to lymphnodes(P<0.05). It was implied through the present study that there was a significant positive correlation between the expression of E-cad and CD44v6(P<0.01). Conclusion Decreased E-cad expression might be significantly correlated with histopathological grade and lymph nodes metastasis. Additionally, decreased CD44v6 expression may be significantly correlated with the tumor histopathological grade. Then, there must be a relationship between the expression of E-cad and CD44v6 in oral and maxillofacial SCC. Therefore, the combined examination with E-cad and CD44v6 could be applied as a reference to detect the invasion and metasis of the oral and maxillofacial SCC.