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| 瓜子金皂苷己对连二亚硫酸钠致氧糖剥夺/复供诱导PC12细胞凋亡的抑制作用 | |
| 引用本文： | 石瑞丽,胡金凤,孔令雷,牛非,吴苗苗,何鑫,李培锋,陈乃宏. 瓜子金皂苷己对连二亚硫酸钠致氧糖剥夺/复供诱导PC12细胞凋亡的抑制作用[J]. 中国药理学通报, 2013, 29(3) |
| 作者姓名： | 石瑞丽 胡金凤 孔令雷 牛非 吴苗苗 何鑫 李培锋 陈乃宏 |
| 作者单位： | 1. 内蒙古农业大学兽医学院,内蒙古,呼和浩特,010018;中国医学科学院药物研究所,北京,100050;包头医学院生理教研室,内蒙古,包头,014040 2. 中国医学科学院药物研究所,北京,100050 3. 中国医学科学院药物研究所,北京,100050;包头医学院生理教研室,内蒙古,包头,014040 4. 内蒙古农业大学兽医学院,内蒙古,呼和浩特,010018 |
| 基金项目： | 国家自然科学基金资助项目，国家科技重大专项 |
| 摘 要： | 目的观察瓜子金皂苷己(polygalasaponin F,PGSF)对氧糖剥夺/复供(oxygen-glucose deprivation and reperfusion,OGD/R)所诱导的PC12细胞凋亡的作用及其机制。方法以连二亚硫酸钠合并无糖Earle’s液造成氧糖剥夺,继而恢复为完全培养基以建立体外OGD/R模型,以Hoechst33342/PI双染及流式细胞术观察细胞受损和凋亡情况;以JC-1荧光染色法测定细胞线粒体膜电位(mitochondrial membranepotential,MMP);以Western blot法检测凋亡相关蛋白的表达。结果 PGSF可明显改善细胞形态并降低细胞受损和凋亡百分率,抑制MMP水平的降低,增加Bcl-2/Bax表达比值。结论 PGSF对OGD/R诱导的PC12细胞凋亡具有明显抑制作用,机制与其调节Bcl-2和Bax的表达及稳定MMP有关。 |
| 关 键 词： | 瓜子金皂苷己 连二亚硫酸钠 氧糖剥夺/复供 PC12细胞 凋亡 线粒体膜电位 Bcl-2 Bax |
Inhibitory effect of Polygalasaponin F on the apoptosis of PC12 cells induced by sodium dithionite-caused oxygen-glucose deprivation and reperfusion |
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| SHI Rui-li , HU Jin-feng , KONG Ling-lei , NIU Fei , WU Miao-miao , HE Xin , LI Pei-feng , CHEN Nai-hong. Inhibitory effect of Polygalasaponin F on the apoptosis of PC12 cells induced by sodium dithionite-caused oxygen-glucose deprivation and reperfusion[J]. Chinese Pharmacological Bulletin, 2013, 29(3) | |
| Authors: | SHI Rui-li HU Jin-feng KONG Ling-lei NIU Fei WU Miao-miao HE Xin LI Pei-feng CHEN Nai-hong |
| Abstract: | Aim To study the effect of Polygalasaponin F(PGSF) on apoptosis of PC12 cells induced by oxygen-glucose deprivation and reperfusion(OGD/R) and its mechanism.Methods The OGD/R model was established by first exposing PC12 cells to sodium dithionite(Na2S2O4) in glucose-free Earle's solution and then by changing the culture into complete mediums,with the cell injury and apoptosis analyzed by Hoechst 33342 / PI dual staining and flow cytometry assay.The mitochondrial membrane potential(MMP) was determined by JC-1 staining,and the expression of Bcl-2 and Bax was detected by Western blot.Results PGSF could significantly improve the morphology of injured PC12 cells and reduce the percentage of apoptotic cells,reverse the decrease in MMP and up-regulate the ratio of Bcl-2/Bax proteins.Conclusion PGSF can markedly inhibit the OGD/R-induced apoptosis of PC12 cells by regulating the expression of Bcl-2 and Bax proteins and maintaining the normal MMP. |
| Keywords: | Polygalasaponin F Na2S2O4 OGD/R PC12 cells apoptosis MMP Bcl-2 Bax |
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