山茱萸配伍组分对糖尿病大鼠心血管病变的保护作用

目的通过观察糖尿病大鼠血脂、血管损伤相关因子及病理变化,分析山茱萸配伍组分保护糖尿病心血管病变的作用机制。方法链脲佐菌素(STZ)复合高脂饲料造成糖尿病大鼠模型,取造模成功大鼠按血糖值随机分组,设立模型组、格列美脲(GLMN)组(0.4 mg.kg-1)、山茱萸配伍组分低剂量(PC-L)组(60 mg.kg-1)、高剂量(PC-H)组(120mg.kg-1),灌胃给药12周,另设空白对照组,模型组与空白组大鼠以蒸馏水灌胃。试剂盒测定血清中TC、TG、HDL-C、LDL-C、NO、T-NOS;放射免疫测定血清中TXB2和6-keto-PGF1α及血浆中ET含量。取大鼠心脏并计算脏器系数,同时观察大鼠心脏和胸主动脉病理组织学变化。结果 PC-L、PC-H组大鼠血清中TC、TG、LDL-C、TXB2含量减少,LDL-C/HDL-C、TXB2/6-keto-PGF1α值降低,与模型组比较差异有显著性(P<0.05,P<0.01)。PC-L组NO、T-NOS含量有增加的趋势,而ET含量减少、NO/ET值升高,与模型组比较差异有显著性(P<0.05);PC-H组NO、T-NOS含量增加、ET含量减少、NO/ET值升高,但与模型组比较差异无显著性。结论山茱萸配伍组分可调节血脂,恢复血管收缩与舒张的动态平衡,改善血液流变性和减轻心脏及胸主动脉病变,具有保护糖尿病大鼠心血管病变的作用。

Protective effects of the Cornus officinalis compatibility of components on cardiovascular lesion in diabetic rats

Aim To study the mechanism of the Cornus officinalis compatibility component's protective effect on the cardiovascular lesion in diabetic rats,by observing blood fat,factors involved in vascular injury and pathological change.Methods Diabetic model was set up in rats by the intake of STZ combined high fat diet.The rats were randomized to model,GLMN(0.4 mg·kg-1),PC-L(60 mg·kg-1),PC-H(120 mg·kg-1) groups by the level of fasting blood glucose and i.g.for 12 weeks.Normal group was set as control.The model and normal group rats were administered with water.The level of TC,TG,HDL-C,LDL-C,NO,T-NOS in serum was examined by kit.TXB2,6-keto-PGF1α in serum and ET in plasma were assayed by radioimmunoassay.Rat heart was taken the organ index was calculated.The change of histopathology in rat heart and thoracic aorta was observed.Results The level of TC,TG,LDL-C,TXB2,and the ratio of LDL-C/HDL-C,TXB2/6-keto-PGF1α in serum of PC-L,PC-H group significantly decreased(P<0.05,P<0.01) compared to those of model group.The level of NO,T-NOS of PC-L group had an increasing trend,while ET decreased and NO/ET increased,which had a significant difference(P<0.05) compared to model group.In PC-H group,the level of NO,T-NOS and the ratio of NO/ET increased;ET decreased.However,there was no significant difference compared to model group.Conclusion The Cornus officinalis compatibility components have a protective effect on cardiovascular lesion in diabetic rats,by regulating blood fat,recovering blood vessel's dynamic balance of constriction and relaxation,improving blood rheological characteristic,and relieving pathological changes in heart and thoracic aorta.