血清可溶性P-选择素、一氧化氮及超敏C-反应蛋白在2型糖尿病大血管病变中的关系

目的 探讨2型糖尿病(T2DM)患者大血管病变与P-选择素、一氧化氮(NO)及超敏C-反应蛋白(hs.CRP)的关系.方法 测定125例2型糖尿病组(60例合并大血管病变,65例无大血管病变)及55例正常对照组患者空腹静脉血中P-选择素(采用酶联免疫吸附试验ELISA)、NO(采用硝酸酶还原法)及hs-CRP(采用免疫散射比浊法)水平.结果 大血管病变组血清P-选择素(350.6±89.2 ng/L)及hs-CRP(5.31±2.67 mg/L)显著高于无大血管病变组(209.3±72.6)ng/L、(2.98±1.56)mg/L]和对照组(96.3±49.2)ng/L、(0.88±0.73)mg/L],大血管病变组血清NO水平(51.26±15.78 μmol/L)显著低于无大血管病变组(63.58±13.64)μmoL/L]和对照组(80.85±15.10)μmoL/L];P-选择素与hs-CRP、收缩压(SBP)及低密度脂蛋白胆固醇(LDL-C)呈正相关(r分别为0.276、0.287、0.289,均为P<0.05),与NO呈负相关(r=-0.271,P<0.05).结论 P-选择素、hs-CRP、LDL-C及收缩压是导致T2DM患者大血管病变的独立危险因素.对T2DM患者检测血清 P-选择素及NO水平并及早干预,可早期预防和延缓大血管并发症的出现.

Detection of P-selection,nitric oxide,and high-sensitive-C-reactive protein in type 2 diabetes mellitus with macroangiopathy and their significance

Objective To explore the relationship between P-selection,nitric oxide(NO),high-sensitive-C-reactive protein(hs-CRP)and type 2 diabetes mellitus(T2DM)with macroangiopathy.Methods In sernm of 125 T2DM subjects(60 subjects with macroangiopathy and 65 subjects without macroangiopathy)and 55 normal controls,the activity of P-selection,NO and hs-CRP was detected by ELISA,Nitrate Reductase Assay and scattering immunoturbidimetric method,respectivley.Results The activity of serum P-selection and hs-CRP was significantly higher in diabetic macroangiopathie group than in non macroangiopathic group and control normal.Serum NO was significantly lower in diabetic rnacroanglopathic group than in non macroangiopathic group and control normal.Serum P-selection was positively correlated with hs-CRP-systolic blood pressure(SBP),LDL-C,and was negatively correla- ted with NO:P-selection,hs-CRP,LDL-C and SBP were independently risk factors for T2DM with macroangiopathy. Conclusion Determination of P-selection and NO is very important for prevention and diagnosis of diabetic mac- roangiopathy in early stage.