| 用绿色荧光蛋白报告肺炎链球菌的体内转化 |
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| 引用本文： | 张雪梅,尹一兵,陈保德,孟江萍,蓝锴,黄远帅,康格非.用绿色荧光蛋白报告肺炎链球菌的体内转化[J].第四军医大学学报,2005,26(20):1847-1850. |
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| 作者姓名： | 张雪梅 尹一兵 陈保德 孟江萍 蓝锴 黄远帅 康格非 |
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| 作者单位： | 重庆医科大学医学检验系临床生化教研室,重庆400016 |
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| 摘 要： | 目的: 了解肺炎链球菌(S.pn)在宿主体内转化发生的情况,以便于深入研究体内细菌转化对其毒力的影响. 方法: 通过基因重组和转化构建绿色荧光报告细菌转化发生的S.pn菌株22G;通过酶切、转化实验、RT-PCR、测序等多种方法鉴定菌株构建成功;通过荧光显微镜观察荧光. 结果: 荧光蛋白可以报告细菌转化的发生;体内实验表明,细菌在宿主体内能够发生转化. 结论: 利用荧光报告细菌转化发生可以用于观察细菌体内转化发生的研究.
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| 关 键 词： | 链球菌 肺炎 转化 细菌 绿色荧光蛋白 |
| 文章编号： | 1000-2790（2005）20-1847-04 |
| 收稿时间： | 2005-03-02 |
| 修稿时间： | 2005-06-04 |
Transformation of Streptococcus pneumoniae in vivo by green fluorescence protein |
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| ZHANG Xue-Mei, YIN Yi-Bing, CHEN Bao-De, MENG Jiang- Ping, LAN Kai, HUANG Yuan-Shuai, KANG Ge-Fei.Transformation of Streptococcus pneumoniae in vivo by green fluorescence protein[J].Journal of the Fourth Military Medical University,2005,26(20):1847-1850. |
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| Authors: | ZHANG Xue-Mei YIN Yi-Bing CHEN Bao-De MENG Jiang- Ping LAN Kai HUANG Yuan-Shuai KANG Ge-Fei |
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| Institution: | Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | AIM: To study when and where streptococcus pneumoniae (S.pn) forms transformation in vivo so as to study the influence of pneumococcal transformation on its virulence. METHODS: S.pn 22G whose transformation forming could be reported by enhanced green fluorescence protein (EGFP) was constructed and was identified by restricted enzyme digestion, transformation experiment, RT-PCR and DNA sequencing. Fluorescence was observed by fleorescence microscope. RESULTS: The fluorescence emitted by 22G which was isolated from BABL/c mice or cultured in vitro was observed. CONCLUSION: S.pn can form transformation in vivo and using EGFP as report protein, pneumococcal transformation forming can be detected in vivo. |
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| Keywords: | Streptococcus pneumoniae transformation bacterial green fluorescence protein |
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