新生儿细菌感染时IL-8 IL-10 IL-13水平的研究及其临床意义

目的新生儿因处于暂时性的免疫功能低下的状态而容易发生感染性疾病,也是新生儿发病和死亡的重要原因.寻找指标以早期诊断新生儿感染性疾病是临床和研究的重点之一.本研究探讨血清IL-8、IL-10、IL-13水平在新生儿细菌感染的早期诊断和疗效判断中的意义.方法用ELISA测定3组血清各细胞因子的水平.感染组:21例细菌感染的足月新生儿.非感染组:20例非感染性疾病的足月新生儿.脐血组:30例正常足月新生儿.结果感染组IL-8、IL-10和IL-13水平(87.0±82.6,35.1±34.8,23.2±46.2 pg/ml)较非感染组升高(56.6±13.2,21.6±12.9,12.0±32.3 pg/ml)(P<0.05);感染组治疗后IL-8和IL-10水平(51.2±3.1,18.5±3.3 pg/ml)较治疗前下降(P<0.05);非感染组IL-13较脐血组(1.2±0.3 pg/ml)显著升高(P<0.05),IL-8、IL-10在两组间无区别.结论新生儿细菌感染时血清IL-8、IL-10和IL-13显著升高,可做为新生儿细菌感染的参考标志物,而IL-8和IL-10的变化有助于评估新生儿感染的治疗效果.

Detection of serum levels of IL-8,IL-10 and IL-13 in neonates with bacterial infection

Objective Neonates are susceptible to infectious diseases and are associated with high mortality due to transient low immunity. This study aims to assess the significance of serum levels of IL-8, IL-10 and IL-13 in early diagnosis and therapy of neonatal infectious diseases in term neonates. Methods Three groups were studied: 1) an Infected group consisting of 21 term neonates with proven bacterial infection; 2) a Non-infected group consisting of 20 sick but non-infected term neonates; and 3) a Umbilical blood group consisting of 30 healthy term neonates from whom umbilical vein blood was obtained immediately after birth. Serum levels of IL-8, IL-10 and IL-13 were examined using enzyme-linked immunosorbant assay. Results The serum levels of IL-8, IL-10 and IL-13 were significantly higher in the Infected group (87.0±82.6, 35.1±34.8 and 23.2±46.2 pg/ml) compared with Non-infected group (56.6±13.2, 21.6±12.9 and 12.0±32.3 pg/ml) (all P<0.05). The serum levels of IL-8 and IL-10 in the Infected group decreased significantly after anti-infection treatment (51.2±3.1 and 18.5±3.3 pg/ml) (both P<0.05). There was no significant difference in the IL-13 level before and after treatment. Serum IL-13 level was significantly higher in the Non-infected group compared to the Umbilical blood group (1.2±0.3 pg/ml) (P<0.05), although the IL-8 and IL-10 levels were the same for the two groups. Conclusions Serum IL-8, IL-10 and IL-13 may be useful as indicators of neonatal bacterial infection. Serum IL-8 and IL-10 might be useful in the evaluation of efficacy of therapy in bacterial infection.