青藤碱对吗啡依赖离体豚鼠回肠作用的实验研究

目的 探讨青藤碱对吗啡依赖性的戒断作用及机制。方法 建立豚鼠离体回肠模型，以纳络酮催促吗啡依赖性戒断收缩，观察10、50和250μmol/L Sin及0．1μmol/L尼莫地平在加入纳络酮前后给药对戒断性收缩的影响；观察青藤碱对乙酰胆碱引起的正常豚鼠回肠收缩的作用。结果 青藤碱及尼莫地平均可抑制纳络酮催促的戒断性收缩，对乙酰胆碱引起的正常豚鼠回肠收缩也有抑制作用，青藤碱的作用呈剂量依赖趋势。结论 青藤碱能够抑制吗啡依赖的戒断症状。

Effect of sinomenine on morphine dependence in isolated guinea pig ileum.

OBJECTIVE: To investigate the effects of sinomenine on morphine withdrawal response and acetylchline (Ach)-induced contracture in isolated guinea pig ileum. METHODS: The withdrawal contracture was elicited by subjecting isolated ileum incubated with morphine (3 micromol/L) at 37.5 degrees Celsius for 4 h to naloxone (1 micromol/L) treatment. Sinomenine (10, 50, 250 micromol/L) and nimodipine (Nim, 0.1 micromol/L) were administered 1 min before and after naloxone in morphine-dependent ilea bathed in Krebs solution containing morphine, to observe the changes in the withdrawal contracture of the ileum. The effect of sinomenine (10, 50, 250 micromol/L) on the contracture of untreated ileum in Krebs solution elicited by acetylcholine was also observed. RESULTS AND CONCLUSION: Naloxone-induced withdrawal contracture or acetylcholine-induced contracture of the ileum was significantly decreased in a dose-dependent manner, indicating that sinomenine can inhibit morphine withdrawal symptoms in guinea pigs.