| 吉非替尼对人肺癌细胞A549生长的影响 |
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| 引用本文： | 姜学东,朱江,刘长浩,全利国,王国柱. 吉非替尼对人肺癌细胞A549生长的影响[J]. 武警医学, 2012, 23(5): 392-394 |
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| 作者姓名： | 姜学东 朱江 刘长浩 全利国 王国柱 |
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| 作者单位： | 110034 沈阳，武警辽宁总队医院：1.胸外科，2.医务处 |
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| 摘 要： |
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| 关 键 词： | 吉非替尼 肺癌 增殖 凋亡 信号转导和转录激活因子 |
| 收稿时间： | 2011-12-20 |
Effect of gefitinib on growth of human lung cancer cell line A549 |
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| JIANG Xuedong , ZHU Jiang , LIU Changhao , QUAN Liguo , WANG Guozhu. Effect of gefitinib on growth of human lung cancer cell line A549[J]. Medical Journal of the Chinese People's Armed Police Forces, 2012, 23(5): 392-394 |
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| Authors: | JIANG Xuedong ZHU Jiang LIU Changhao QUAN Liguo WANG Guozhu |
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| Affiliation: | 1.Department of Thoracic Surgery,
2.Department of Medical Service, Liaoning Provincial Corps Hospital, Chinese People’s Armed Police Forces, Shenyang 110034, China |
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| Abstract: | Objective To investigate the effect of gefitinib on the growth of human lung cancer cell line A549 and its molecular machenism.Methods The growth suppressing effect of gefitinib on A549 was quantified by MTT assay.Apoptosis was assayed with annexinⅤ-FITC/PI doubled stained flow cytometry.Western blotting assay was adopted to identify the expression of STAT3.Results After treatment with gefitinib,MTT showed that cell proliferation was inhibited,and the highest inhibitory rate was(80.20±0.45)%(P<0.05).AnnexinⅤ-FITC/PI doubled stained flow cytometry showed that the cell apoptosis rate was(41.39±1.74)%,rising remarkably(P<0.05).Western blotting showed significant decline of expression of STAT 3.Conclusions Gefitinib can significantly inhibit the growth of human lung cancer cell line A549 and induce early apoptosis.The effect may be due to the down-regulation of STAT 3 expression. |
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