尿激酶型纤溶酶原激活物及其Ⅰ型抑制剂mRNA在实验性肌炎肌肉组织中的表达和意义

 目的 探讨尿激酶型纤溶酶原激活物（urokinase type plasmin-ogen activator，uPA）及其Ⅰ型抑制剂（plasminogen activator inhibitor,PAI-1）mRNA 在豚鼠实验性自身免疫性肌炎(experimental autoimmune myositis,EAM)肌肉中的表达及其意义。 方法 利用兔骨骼肌肌球蛋白加完全弗氏佐剂多次免疫豚鼠，同时联合腹腔注射百日咳毒素，制成EAM模型，观察其临床表现、血CK、肌肉病理的改变；用RT-PCR 方法检测11例EAM组模型成功豚鼠肌肉uPA 和PAI-1基因mRNA的表达，以10 例正常豚鼠的肌肉作为对照组。 结果 （1）EAM组肌肉中uPA、PAI-1基因mRNA的表达均较正常组显著增高（ P < 0.01) ；（2）肌肉中uPA与PAI-1基因mRNA表达的比例在正常组和EAM组无统计学差异( P <0.01)。 结论 uPA、PAI-1可能参与了EAM的发病机制。

Expression and significance of gene mRNA of urokinase type plasminogen activator and its inhibitor 1 type in muscle of exprimental autoimmune myositis guinea pigs

Objective To study the expression and significance of gene mRNA of urokinase type plasminogen activator and its inhibitor 1type(PAI-1)in the muscle of exprimental autoimmune myositis(EAM) guinea pigs.Methods The EAM model was established by repeatedly immunizating guinea pigs with rabbit skeletal muscle myosin plus Freund’s complete adjuvant and giving these pigs a celiac injection of pertussis toxin.The changes in clinical symptoms,serum creatine kinase and pathology were observed.The expression level of uPA and PAI-1 in the skeletal muscle was detected by RT-PCR and compared between EAM group(n=11) and normal group(n=10).Results(1) The level of expression of the uPA and PAI-1 mRNA in the muscle increased significantly in EAM group,compared with normal group(P<0.01).(2)There was no difference in the ratio between expression of the uPA and PAI-1 mRNA in the muscle tissues of EAM group and normal group(P<0.01).Conclusions uPA and PAI-1 may contribute to the pathogenesis of EAM.