1，3-丁二烯致染色体损伤的遗传易感性与CYP2E1和GSTs基因多态性关系

[目的]探讨代谢酶基因CYP2E1和GSTs的基因多态性与1，3-丁二烯致外周血淋巴细胞染色体损伤易感性的关系。[方法]采用胞质分裂阻滞微核试验方法（CBMN）评价166名丁二烯接触工人和41名对照组染色体损伤水平，应用PCR-RFLP测CYP2E1 clc2基因多态，PCR法测GSTT1和GSTM1缺失情况。[结果]接触组和对照组的微核发生率分别为（3．23±2．49）％。和（1．22±1．19）％。，差别有统计学意义（P〈0．01）。多因素Poisson回归分析发现CYP2E1 c1c2／c2c2基因型和GSTMl非缺失型与染色体损伤相关（分别为矿=14．39，P〈0．01和χ^2=4．23，P〈0．05）。没有发现年龄、工龄、性别、吸烟和饮酒等与微核率之间的关系。[结论]双核淋巴细胞微核数可以作为1，3．丁二烯接触早期健康损害的指标。CYP2E1 c1c2／c2c2基因型、GSTM1非缺失型与1,3-丁二烯诱导的染色体损伤有关。

Study on the Relationship between Chromosomal Damage and Polymorphisms of Metabolizing Enzymes in 1,3-butadiene-exposed Workers

[ Objective ] To explore the relationship between chromosomal damage induced by 1,3-butadiene （ BD ） and polymorphisms of metabolizing enzymes of CYP2E1 and GSTs. [ Methods ] Cytokinesis-block micronucleus （ CBMN ） assay was used to detect chromosomal damage in peripheral lymphocytes of 166 1,3-butadiene exposed workers and 41 controls. PCR-RFLP technique was applied to detect polymorphisms in CYP2E1 clc2. Null genotypes of GSTT1 and GSTMI were detected by PCR. [ Results ] Compared the CBMN frequencies of the two groups we found that the frequency in exposed workers was higher than that in control group（ P〈0.01 ）. Individuals with the CYP2E1 c1c2/c2c2 and GSTMI positive genotype were observed to have significantly higher CBMN frequencies than individuals with more common genotypes （Z~=14.39, P〈0.01 and χ^2=4.23, P〈0.05 ）. Sex, age, duration of work, smoking and drinking habit had no relationship with CBMN frequencies. [ Conclusion ] BD-exposed workers had higher risk of chromosomal damage compared with control. Cytokinesis-block micronucleus （ CBMN ） assay can be used to evaluate the early damage of butadiene-exposed workers. CYP2E1 c1c2/c2c2 and GSTMI positive genotype showed higher average CBMN frequencies than those with more common genotypes.