Assay for heat-labile Escherichia coli enterotoxin using sandwich erythroimmunoassay |
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Authors: | Y. Germani E. Begaud J. L. Guesdon |
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Affiliation: | (1) Unité de Bactériologie Expérimentale, Institut Pasteur, BP 61, Nouméa, New Caledonia;(2) Laboratoire des Sondes Froides, Institut Pasteur, 28 rue du Dr. Roux, F-75 724 Paris Cedex 15, France |
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Abstract: | We investigated the possibility of using a sheep erythrocyte-antibody conjugate as reagent in a sandwich erythroimmunoassay (SERIA) procedure to detect and titrate Escherichia coli heat-labile enterotoxin (LT) with the naked eye. In this assay, which is based on the immunological similarity between Vibrio cholerae toxin (CT) and LT, rabbit anti-CT IgG was used as immunosorbent, and sheep erythrocytes, sensitized with the rabbit anti-CT antibodies, were used as indicator. The sensitivity of the test was demonstrated by a comparative study using an enzyme-linked immunosorbent assay (ELISA). The results obtained by SERIA with 130 samples correlated well with those of a Vero cell assay and a GM1-ELISA. The test is easy, relatively cheap and as sensitive as other standard techniques; it is particularly suited for field laboratories, especially in tropical countries, and a large number of strains may be examined daily. |
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