海洋贝类综合提取物对实验性动脉粥样硬化的治疗作用

目的研究海洋贝类综合提取物对实验性动脉粥样硬化的治疗作用。方法先建立高脂食饵性鹌鹑动脉粥样硬化模型，然后经口服连续给予5、10及20g/kg海洋贝类综合提取物，于用药第2、4周末测定血清及主动脉和心肌中脂质含量，并对主动脉、冠状动脉和肝脏进行肉眼及光镜组织学检查。结果用药2周后5、10及20g/kg海洋贝类综合提取物均可显著降低血清甘油三酯水平（P〈0．05及P〈0．01），20g/kg海洋贝类综合提取物组血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平明显降低，高密度脂蛋白胆固醇水平明显升高（P〈0．05）；用药4周后，5、10及20g/kg海洋贝类综合提取物组血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平明显降低（P〈0．05及P〈0．01），高密度脂蛋白胆固醇水平明显升高（P〈0．05及P〈0．01）；主动脉壁及心肌组织中总胆固醇、甘油三酯含量也明显降低（P〈0．05及P〈0．01）。病理检测结果发现鹌鹑冠状动脉、主动脉内膜粥样硬化斑块的病变程度较模型组明显减轻。结论海洋贝类综合提取物治疗用药具有降低高血脂，促进动脉粥样硬化消退作用。

Effects of Comprehensive Extract from Maritine Shellfish Experimental Atherosclerosis in Quails

Aim To study the therapeutic action of extract of maritine shellfish (EMS) on experimental atherosclerosis in quails. Methods The experimental atherosclerosis model in quails was induced exogenously by hyperlipidic feed for 8 weeks. Then EMS was orally given. The influence of EMS on lipid level in serum was detected with enzyme at the second and fourth weekend after administration. The effects of EMS on the atherogenic plaque in artery and fatty liver were observed by histopathological methods. Results At the second weekend after administration EMS 5, 10 and 20 g/kg significantly reduced the level of triglyceride (TG) in serum (p<0.05, p<0.01). EMS 20 g/kg evidently lowered the serum total cholesterol (TC) and low density lipoprotein cholesterol (LDLC). The high density lipoprotein cholesterol (HDLC) concentration was raised (p<0.05). In therapeutic oral administration for 4 weeks EMS 5, 10 and 20 g/(kg·d) markedly reduced the volume of TG, TC, LDLC in serum. At the same time, TC and TG in the myocardial and aortic wall were reduced (p<0.05, p<0.01). The serum HDLC was increased (p<0.05, p<0.01). Compared with model group, the degree of atherosclerotic lesion in aorta and coronary artery and fatty degeneration of liver in EMS group was reduced (p<0.05). Conclusion EMS shows action of the regression for atheroscleritic lesion and the regulation of hyperlipidemia in therapeutic administration.