黄体酮对戊四唑致痫大鼠海马内微管相关蛋白2免疫反应的影响

目的：研究黄体酮(P)对戊四唑(PTZ)致痫大鼠的发作情况和对海马结构内的微管相关蛋白2(MAP2)的影响。方法：采用成年去卵巢(OVX)雌性SD大鼠，设立空白对照组，实验对照组，实验给药组，取含背侧海马的脑片，进行免疫组化染色。结果：(1)实验给药组大鼠均未出现癫痫发作，实验对照组大鼠发作均达Ⅳ级以上。(2)实验对照组海马结构内MAP2免疫阳性反应减弱，而实验给药组则增强。结论：P可对抗PTZ致痫的行为发作，并且上调了海马内MAP2的免疫活性，可能参与了P对抗癫痫的机制。

Effects of progesterone on microtubule associated protein 2 in hippocampus of pentylenetetrazol induced epileptic rats

Objective: To explore the effects of p rogesterone on pentylenetetrazol (PTZ) induced seizures and microtubule associated protein 2 (MAP2) of the hippocampus in ovariectomized female rats. Methods:Ovariectomized (OVX) adult females SD rats were randomly divided into NS control, experimental control and experimental groups. The brains were removed and slices were cut coronally using a vibrator. The slices with dorsal hippocampus were stained by ABC immunohistochemistry method. Results:(1)Pretreatment with progesterone protected OVX rats from PTZ-induced seizures completely, while rats of experimental control group all exhibited stage 4 or 5 seizures; (2) MAP-IR decreased in Hilus, molecular layer of dentate gyrus and CA1 of experimental control group compared with NS+NS group. However, compared with those of NS control group. MAP-IR increased in experimental group. Conclusion:Progesterone can protect OVX rats against PTZ-induced seizures, and it may enhance MAP2 immunohistochemistry in female rats, which may be one of mechanisms of P's anticonvulsant effects and protective action.