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| 海洋放线菌发酵物对肝癌细胞株生长和凋亡的影响 | |
| 引用本文： | 施珊珊,江振友,魏守一,李春艳,周光雄.海洋放线菌发酵物对肝癌细胞株生长和凋亡的影响[J].广东医学,2009,30(4). |
| 作者姓名： | 施珊珊 江振友 魏守一 李春艳 周光雄 |
| 作者单位： | 1. 暨南大学医学院微生物与免疫学教研室,广州,510632 2. 暨南大学生命科学技术学院生物医学工程系,广州,510632 3. 暨南大学药学院生药学教研室,广州,510632 |
| 基金项目： | 广东省医学科学研究基金 |
| 摘 要： | 目的 研究一株南海海洋放线菌的发酵产物对肝癌细胞生长和凋亡的影响。 方法 采用MTT法评定其对肝癌细胞HepG-2增殖抑制作用及对正常细胞Vero细胞的细胞毒性，经Hoechst 33258 (8g/L)染色进行形态学观察，应用流式细胞术检测细胞周期、凋亡和凋亡基因Fas的表达情况。 结果 发酵物对HepG-2细胞的生长抑制效果明显，荧光显微镜下观察发现培养细胞中出现核固缩、凋亡小体；发酵物在诱导HepG-2细胞凋亡同时，下调Bcl-2表达水平，且均呈剂量效应关系。 结论 发酵物通过阻滞肿瘤细胞的生长及通过抑制Bcl-2诱导细胞凋亡等机制，对肝癌细胞的增殖有一定的抑制作用。 |
| 关 键 词： | 海洋放线菌 MTT 细胞凋亡 |
Effects of marine actinomycete fermented compound on the growth and apoptosis of HepG-2 cells in vitro |
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| SI Shan-shan,JIANG Zhen-you,WEI Shou-yi,LI Chun-yan,ZHOU Guang-xiong.Effects of marine actinomycete fermented compound on the growth and apoptosis of HepG-2 cells in vitro[J].Guangdong Medical Journal,2009,30(4). | |
| Authors: | SI Shan-shan JIANG Zhen-you WEI Shou-yi LI Chun-yan ZHOU Guang-xiong |
| Institution: | SHI Shan-shan,JIANG Zhen-you,WEI Shou-yi,LI Chun-yan,ZHOU Guang-xiong.Department of Microbiology , Immunology,Medical College,Guangzhou 510632,China |
| Abstract: | Objective To investigate the influence of Marine Actinomycete Fermented Compound on the growth and apoptosis of HepG-2 cells in vitro and its possible mechanism. Methods The inhibition of Fermented Compound on HepG-2 cells and normal cells(Vero) proliferation was detected by MTT method and disposed cells were stained with Hoechst 33258 (8 g/L). Cell apoptosis and expression of gene Bcl-2 gene were further analyzed with flow cytometry.Results Fermented Compound significantly inhibited the growth of HepG-2 ce... |
| Keywords: | MTT Bcl-2 |
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