Circulating tumor cells with a putative stem cell phenotype in peripheral blood of patients with breast cancer |
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| Authors: | Panayiotis A. Theodoropoulos Hara Polioudaki Sofia Agelaki Galatea Kallergi Zacharenia Saridaki Dimitris Mavroudis Vassilis Georgoulias |
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| Affiliation: | 1. Department of Biochemistry, School of Medicine, University of Crete, Heraklion, Greece;2. Laboratory of Τumor Cell Biology, School of Medicine, University of Crete, Heraklion, Greece;3. Department of Medical Oncology, University General Hospital of Heraklion, Heraklion, Greece |
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| Abstract: | The CD44+/CD24−/low and ALDH1+ cell phenotypes are associated with stemness and enhanced tumorigenic potential in breast cancer. We assessed the expression of CD44, CD24 and ALDH1 on tumor cells circulating in the peripheral blood (CTCs) of patients with metastatic breast cancer using triple-marker immunofluorescence microscopy. Among a total of 1439 CTCs identified in 20 (66.7%) out of 30 patients, 35.2% had the stem-like/tumorigenic phenotype CD44+/CD24−/low, whereas 17.7% of the CTCs analyzed in seven patients, were ALDH1high/CD24−/low. In conclusion, we report the existence of a subpopulation of CTCs with putative stem cell progenitor phenotypes in patients with metastatic breast cancer. |
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| Keywords: | Micrometastasis Circulating tumor cells Breast cancer Stem cells Triple-marker immunofluorescence |
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