Beta-adrenergic signals regulate adipogenesis of mouse mesenchymal stem cells via cAMP/PKA pathway |
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Authors: | Haifang Li Chichun Fong Yao Chen Guoping Cai Mengsu Yang |
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Institution: | 1. Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China;2. Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China;3. Shenzhen Key Laboratory of Biochip Research, City University of Hong Kong, Shenzhen 518057, PR China;4. Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, PR China |
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Abstract: | The adipogenic capacity of mesenchymal stem cells (MSCs) and the involvement of β-adrenergic signals in lipolysis and thermogenesis have been well established. However, little is known about the development of β-adrenergic receptor (β-AR) systems and the role of β-adrenergic signals in adipogenic differentiation of MSCs. In this study, we demonstrated that both the mRNA and protein levels of β2- and β3-AR were up-regulated following adipogenesis of mouse bone marrow derived MSCs. We also established that β-AR agonists negatively while antagonists positively affected MSC adipogenesis. Both the β2- and β3-AR were involved in MSC adipogenesis, with β3-AR being the predominant subtype. The effect of β-ARs on MSC adipogenesis was at least partly mediated via the cAMP/PKA signaling pathway. These findings suggested that MSC is also a target for β-adrenergic regulation, and β-adrenergic signaling (major β3-signaling) plays a role in MSC adipogenesis. |
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Keywords: | Mesenchymal stem cells (MSCs) Adipogenesis β-Adrenergic receptors (β-ARs) cAMP/PKA pathway |
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