| Abstract: | Interaction of normal and Salmonella typhi `H'-sensitized rat lymph node cells with two different types of anti-lymphocyte serum (ALS) has been studied. One type of ALS, named as ALS(N), was prepared by immunizing rabbits with normal non-sensitized lymph node cells while the other, ALS(I), was produced by injecting rabbits with sensitized cells. The histogram of electrophoretic mobilities (EPM) of ALS(N)-treated normal cells resolves into two Gaussian components corresponding to Class `A' and Class `B' cells respectively. ALS(N) brings about a significant reduction in the mean EPM of both these components indicating that ALS(N) binds to both the functional cell types with equal avidity. Analysis of the histogram of EPM of ALS(N)-treated sensitized cells reveals that ALS(N) leaves the mean EPM of antibody-producing Class `C' cells unchanged. ALS(I), on the other hand, is effective in binding to Class `C' cells in addition to the other cell types. These investigations thus demonstrate that ALS(N) has a differential action on the antigen-sensitive cells as compared to the antibody-producing cells. Further, this lack of interaction with Class `C' cells appears to be a result of a radically different surface-antigenic configuration of these cells. |
