| 中华眼镜蛇毒抗瘤多肽对人卵巢癌HO-8910细胞的增殖抑制和Fas/FasL表达的影响 |
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| 引用本文： | 戚潜辉,陈盛强,宋绿茵,李锦玉.中华眼镜蛇毒抗瘤多肽对人卵巢癌HO-8910细胞的增殖抑制和Fas/FasL表达的影响[J].广州医药,2007,38(1):62-65. |
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| 作者姓名： | 戚潜辉 陈盛强 宋绿茵 李锦玉 |
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| 作者单位： | 1. 广州医学院第二附属医院妇产科,510260
2. 广州医学院第二附属医院神经研究所,510260 |
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| 基金项目： | 广州市中医药中西医结合科研项目
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广东省科技厅社会发展与基础研究项目 |
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| 摘 要： | 目的 探讨中华眼镜蛇毒抗瘤多肽对人卵巢癌HO-8910细胞株的抑制作用及Fas/FasL表达的影响.方法 用MTT比色法检测不同浓度中华眼镜蛇毒抗瘤多肽对HO-8910细胞的增殖抑制,RT-PCR和免疫组织化学观察原癌基因Fas/FasL表达的变化.结果 中华眼镜蛇毒抗瘤多肽对HO-8910细胞增殖抑制作用成剂量依赖性,RT-PCR和免疫组化结果显示,随着中华眼镜蛇毒抗瘤多肽作用剂量的增加,Fas/FasL基因的表达无明显变化.结论 中华眼镜蛇毒抗瘤多肽对人卵巢癌HO-8910细胞系的增殖具有明显的抑制作用,并对原癌基因Fas/FasL的表达无抑制作用.
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| 关 键 词： | 中华眼镜蛇毒抗瘤多肽 人卵巢癌HO-8910细胞 基因 Fas/FasL |
| 修稿时间： | 2007-01-08 |
Inhibition of proliferation and influence of Fas/FasL expression by antitumor polypeptide from naja actra venom in HO-8910 cell |
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| Qi Qianhui,Chen Shegqiang,Song Luyin,Li jinyu.Inhibition of proliferation and influence of Fas/FasL expression by antitumor polypeptide from naja actra venom in HO-8910 cell[J].Guangzhou Medical Journal,2007,38(1):62-65. |
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| Authors: | Qi Qianhui Chen Shegqiang Song Luyin Li jinyu |
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| Institution: | Department of Gynecology and Obstetrics, the Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China |
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| Abstract: | Objective This study was designed to investigate the inhibition of antitumor Polypeptide from Naja Actra Venom(APNAV) on HO-8910 cell line and its mechanism.Methods MTT was used to measure the levels of the proliferation of HO-8910 cultured with APNAV in different concentrations.The effects of apotosis on cell to HO-8910 were observed by DNA Ladder.The expression of Fas/FasL was measured by RT-PCR and immunohistochemical technology.Results The proliferation of HO-8910 was obviously inhibited by APNAV in a dose dependent manner.The outcome showed that the expression of gene Fas/FasL was not notably changed,when the dose of APNAV was increased.Conclusion APNAV can inhibit the proliferation of HO-8910 and can not affect the expression of gene Fas/FasL. |
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| Keywords: | Antitumor polypeptide from Naja Actra Venom HO-8910 cell Gene Fas/FasL |
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