胃肠道黏膜相关淋巴组织淋巴瘤BCL-10的表达

目的　探讨BCL 10蛋白在胃肠道黏膜相关淋巴组织淋巴瘤 (MALToma)中表达的意义。方法　应用CD2 0、CD79a、CD3、CD4 5RO、CD2 3、CD5、CD10单克隆抗体对 4 3例胃肠道MALToma进行检测 ,其中 2 5例为经典MALToma(MALToma LG) ,临床为惰性 ;18例为伴有少数大细胞转化的MALToma(TransformedMALToma)。用SABC免疫组化的方法检测瘤细胞BCL 10蛋白的表达。结果　在 2 5例惰性MALToma标本中 10例为BCL 10核阳性 ,1例为BCL 10核与浆同时阳性 ,3例为瘤细胞浆阳性 ,11例为阴性 ;在 18例转化的MALToma中 7例为BCL 10核阳性 ,1例为BCL 10核与浆同时阳性 ,2例为瘤细胞浆阳性 ,8例为阴性。BCL 10核阳性在胃肠道MALToma中的出现率较高 ,达 4 4 .2 %。结论　BCL 10在胃肠道MALToma中发生率较高 ,该高发生率与肿瘤的发生或演进可能有一定的相关性

Expression of BCL-10 in gastrointestinal mucosa-associated lymphoid tissue lymphoma

Objective To explore the significance of BCL-10 protein expression in the gastrointestinal mucosa-associated lymphoid tissue(MALT) lymphoma. Methods Immunohistochemistry studies were performed using CD20,CD79a,CD3,CD45RO,CD23,CD5,CD10 monoclonal antibodies in 43 cases of gastrointestinal MALT lymphomas, including 25 indolent classical MALT lymphomas and 18 MALT lymphomas with large cell transformation. BCL-10 protein expression was assayed in the tumor cells. Results In 25 low-grade MALT lymphomas, expression of BCL-10 was found in the nuclei in 10 cases, in both nuclei and cytoplasm 1 case, in cytoplasm 3 cases and no expression 11 cases. In 18 transformed MALT lymphomas, BCL-10 was expressed in the nuclei in 7 cases, in both nuclei and cytoplasm 1 case, in cytoplasm 2 cases, no expression 8 cases. The frequency of BCL-10 expression in nuclei was the highest (44.2%). Conclusion The frequency of BCL-10 expression in nuclei in the gastrointestinal MALT lymphoma is high, indicating that it may associate with the pathogenesis of this entity, and may be helpful to its diagnosis.