大豆异黄酮对大鼠实验性粥样硬化动脉壁内粘附分子基因表达的影响

为研究大豆异黄酮在动脉粥样硬化发生过程中与细胞间粘附分子1和血管细胞粘附分子1基因表达之间的关系。将60只大鼠按总胆固醇含量随机分为6组，分别喂饲基础饲料、高脂饲料、高脂饲料加不同剂量大豆异黄酮和高脂饲料加设雌激素。20周后处死动脉，光学显微镜检测HE染色的主动脉壁横切面病理改变，免疫组织化学和Western-blot法检测血管壁内细胞间粘附分子1和血管细胞粘附分子1蛋白，并运行计算机图像分析系统进行组间分析比较。结果发现，大鼠主动脉粥样斑块中，细胞间粘附分子1和血管细胞粘附分子1基因表达明显增加，大豆异黄酮可以减轻高脂饲料诱导的主动脉病理变化，减弱细胞间粘附分子1和血管细胞粘附分子1基因在主动脉内的表达。此结果提示，大豆异黄酮具有抗动脉粥样硬化形成作用，此作者可能是通过减弱粘附分子在主动脉壁的表达来实现。

Soy Isoflavones Inhibits Gene Expression of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 in Aorta Vessel of Atherosclerotic Rat Predisposed with High Cholesterol and High Fat Diets

Aim To investigate high quantity of total soy isoflavones influence adhesion molecule such as intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in aorta vessel during the formation of atherosclerosis in rats predisposed with high cholesterol and high fat diet. Methods 60 Wistar rats were divided in 6 groups randomly, rats of the normal diet control (NDC)group were fed with normal diet. Rats of the remainder groups were fed high cholesterol (3.5%), high fat (10%) and 1% hyocholic salt plus 85% normal diet. Rats of the high fat diet plus low (HLI group), medium (HMI group), high (HHI group) dose ISO groups were fed with high content of total isoflavones simultaneously and the dosage were 30 mg/kg, 90 mg/kg and 270 mg/kg body weights perday; rats of the high fat diet plus estrobene control (HEC) group was given estrobene 0.25 mg/kg body weights perday. After 20 week, rats were killed and aorta were taken. HE staining was used to study the pathology during the formation of atherosclerosis. Body weights and organ weight were detected to explore if there were physical impairmen when isoflavones were given to rats. Immunohistochemistry and Western-blotting were used to detect ICAM-1 and VCAM-1 gene expression in aorta vessel and computer image analysis was used to check the degree of gene expression about VCAM-1 or ICAM-1. Results Soy isoflaones can inhibits atherosclerotic plaque formation dose dependently, lower the high expression of ICAM-1 and VCAM-1 in aorta vessel caused by high cholesterol and high fat diet (Compared to B group P<0.05). Conclusion Soy isoflavones can inhibit expression of adhesion molecules genes such as ICAM-1, VCAM-1 in aortic of rats fed with high cholesterol and high fat diets.