Specific Amino Acid Profile in Culture Media Conditioned by Human Pancreatic Cancer Cell Lines |
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| Institution: | 1. Department of Emergency Medicine, Harbor-UCLA Medical Center, 1000 W. Carson Boulevard, Torrance, CA 90502, USA;2. Department of Emergency Medicine, UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA;3. Department of Emergency Medicine, University of Maryland School of Medicine, 110 South Paca Street, Sixth Floor, Suite 200, Baltimore, MD 21201, USA;1. Laboratory of Molecular Genetics and Cytogenetics, Institute of Biological Sciences, Federal University of Goiás - UFG, Goiânia, GO CEP 74001-970, Brazil;2. Laboratory of Cardiovascular Phisiology, Institute of Biological Sciences, Federal University of Goiás - UFG, Goiânia, GO CEP 74001-970, Brazil;3. Mutagenesis and Microorganisms Radiobiology Laboratory, Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO 74690-900, Brazil;4. Department of Morphology, Institute of Biological Sciences, University Federal of Goiás, Goiânia, GO, Brazil;5. Department of Biomedicine, Pontifical Catholic University of Goiás, Goiânia, GO, Brazil;6. Department of Chemistry, Federal University of São Carlos, SãoCarlos, SP CEP 13.565-905, Brazil;1. The First Clinical College, Wenzhou Medical University, Wenzhou, China;2. Department of Joint Surgery, the Affiliated Hospital of Qingdao University, Qingdao, China;3. Department of Medical Oncology, the First Hospital of China Medical University, Shenyang, China |
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| Abstract: | Background: In malignant diseases, circulating amino acid profiles correlate with organ sites of malignancy. Direct effects of malignant cells on the extracellular amino acid profile are still uncertain. Methods: Free amino acids were measured in serum-free culture media (RPMI1640) conditioned by two human pancreatic cancer cell lines (Panc-1 and HPAF), a hamster pancreatic cancer cell line (PC-1), a human epidermoid carcinoma cell line (A431), and a human fibroblastic cell line (Ag-1523). Nonconditioned RPMI-1640 medium was used as control. Results: Amino acid profiles were changed in all the conditioned media, caused by a decrease or increase in the original amino acids and by the appearance of amino acids that were not present in non-conditioned medium. Media conditioned by two human pancreatic cancer cell lines showed similar amino acid profiles, which were characterized by a decrease in glutamine, cysteine and serine, increase in glycine, proline and glutamic acid and appearance of ornithine and alanine. Conclusion: Culture media show changed amino acid profiles following incubation with cell lines of pancreatic or non-pancreatic origins. Different human pancreatic cancer cell lines cause similar changes in amino acid profiles of media. |
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