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| RP-HPLC法测定小柴胡颗粒中黄芩苷含量 | |
| 引用本文： | 沈静,刘峰,张春来,赵向阳. RP-HPLC法测定小柴胡颗粒中黄芩苷含量[J]. 安徽中医学院学报, 2001, 20(1): 48-49 |
| 作者姓名： | 沈静 刘峰 张春来 赵向阳 |
| 作者单位： | 1. 江苏省连云港康缘制药有限责任公司，江苏 连云港 222001 2. 安徽省滁州卫生学校 3. 安徽省合肥利民制药厂 4. 安徽省宿州地区药检所 |
| 摘 要： | 目的 :以RP HPLC测定小柴胡颗粒中黄芩苷含量。方法 :黄芩苷以超声法直接用甲醇提取。色谱条件为 :AlltimaC18色谱柱 ( 2 50mm× 4 .6mm ,5μm ) ;流动相为甲醇 水 磷酸 ( 60∶4 0∶0 .2 ) ;柱温室温 ;检测波长 2 80nm。结果 :黄芩苷在 0 .0 6～ 0 .60 μg线性关系良好 ,平均加样回收率为 10 0 .2 3% ,RSD =1.70 % (n =5)。结论 :本法快速、准确、重现性好 ,可用于本制剂质量控制。 |
| 关 键 词： | 小柴胡颗粒 黄芩苷 RP－HPLC 测定 |
| 文章编号： | 1000-2219(2001)01-0048-02 |
| 修稿时间： | 2000-10-04 |
QUANTITATIVE DETERMINATION OF BAICALIN IN XIAOCHAIHU GRANULE BY RP-HPLC |
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| SHEN Jing,LIU Feng,ZHANG Chun-lai,ZHAO Xiang-yang. QUANTITATIVE DETERMINATION OF BAICALIN IN XIAOCHAIHU GRANULE BY RP-HPLC[J]. Journal of Anhui Traditional Chinese Medical College, 2001, 20(1): 48-49 | |
| Authors: | SHEN Jing LIU Feng ZHANG Chun-lai ZHAO Xiang-yang |
| Abstract: | Objective：Quantitative determination of Baicalin in Xiaochaihu Granule by RP-HPLC.Method:Baicalin was extracted with methanol by an ultrasonic wave.Chromatographic conditions were as follows:Alltima C18 column(250mm×4.6mm，5μm)；Mobile phase:methanol-water-phosphoric acid(60∶40∶0.2)；Column tempareture:the temperature of laboratory;Detection wavelength:280nm.Result:The standard curve was linear in the range of 0.06～0.60μg.The average recovery rate and RSD were 100.23% and 1.70% respeclively.(n=5).Conclusion:This method is rapid,reliable.It is suitable for quality control of the production. |
| Keywords: | Xiaochaihu Granule Baicalin RP-HPLC |
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