弱电场下博来霉素对人头颈部肿瘤裸鼠移植瘤CyclinD1抑制作用的研究

目的1）研究博来霉素抑制CyclinD1表达的作用。2）探索弱电场下电穿孔促进博来霉素对头颈部肿瘤的治疗效果。方法采用免疫组化的方法对人头颈部癌裸鼠移植瘤的肿瘤组织切片（40）张进行CyclinD1检测。其中AB为电化学组A：50v（E），20mg（B）；B：100v（E），20mg（B）；C：药物组：20mg（B）；DE电压组；D：50v；E：100v；F：为对照组予以PBS。结果A、B、C组的CyclinD1的表达率明显低于D、E、F组。其中A的表达率为21．10±7．11％；B的表达率为5，60±3．05％；C的表达率为37．43±14．53％；D的表达率为49．64±4．59％；E的表达率为41．74±5．27％；F的表达率为59．27±3．79％。药物治疗组（A、B、C）与对照组F的CyclinD1表达差异具有统计学意义（P〈0．01）。电化学组（AB）与单纯药物组（C）CyclinD1表达差异具有统计学意义（P〈0．01）。结论1）博来霉素可以抑制CyclinD1表达。2）弱电场下电穿孔促进了博来霉素对头颈部肿瘤的治疗效果。

Repressing effect to Cyclin D1 of bleomycin by low-voltage in vivo electroporation: a study of human head and neck carcinorma in nude mice

Objective 1. To study the repressing effect of bleomycin to Cyclin D1 . 2. To discuss the enhancement of antitumor effect of blemycin by low--voltage in vivo electroporation. Methods Forty slices were classed as A：50v（E）,20mg（B）;B：100v（E）,20mg（B）; C：20mg（B） ;D：50v; E：100v ; F （PBS） was taken as control. Cyclin D1 was detected by using the method of immunohistochemisty. Results The expression of Cyclin D1 in A, B, C was obviously lower than D, E, F. The expression of A was 21.10±7.11% ;B was 5.60±3.05; C was 37.43±14.53; D was 49.64±4.59; E was 41. 74±5. 27; F was 59. 27±3. 79. The positive expressions of Cyclin D1 were related to histological grade. Conclusions 1） Bleomycin can repress the expression of Cyclin D1. 2） Electroporation. by low-voltage have effect on the development of treatment.