| 转化生长因子β1和p27^kip1mRNA在胃粘膜癌变中的变化 |
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| 引用本文： | 李景和,文继舫.转化生长因子β1和p27^kip1mRNA在胃粘膜癌变中的变化[J].湖南医科大学学报,2000,25(3):259-261. |
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| 作者姓名： | 李景和 文继舫 |
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| 摘 要： | 探讨转化生长因子β及ｐ２７＾ｋｉｐ１在胃癌癌变过程中的作用。方法：采用逆转录ＰＣＲ技术分别检测胃粘膜正常组织、单纯性增生、不典型增生及胃癌组织标本中ＴＧＦβ１和ｐ２７＾ｋｉｐ１ｍＲＮＡ表达水平。结果：正常胃粘膜、单纯性增生、不同共型增生及胃癌组织均有ＴＧＦβ１和ｐ２７＾ｋｉｐ１ｍＲＮＡ表达，但ＴＧＦβ１和ｐ２７ｋｉｐ１的ｍＲＮＡ表达水平在上述４种组织中均依次逐渐下降，胃癌组的表达水平最低，与其它三
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| 关 键 词： | 胃粘膜癌变 TGFβ1 P27^KIP1 mRNA |
Alterations of transforming growth factor beta 1 and p27kip1 mRNAs in gastric mucosa carcinogenesis] |
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| J H Li,J F Wen,J Xu.Alterations of transforming growth factor beta 1 and p27kip1 mRNAs in gastric mucosa carcinogenesis][J].Bulletin of Hunan Medical University,2000,25(3):259-261. |
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| Authors: | J H Li J F Wen J Xu |
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| Institution: | Department of Pathology, Hunan Medical University, Changsha 410078. |
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| Abstract: | OBJECTIVE: To investigate the effect of transforming growth factor beta 1 (TGF beta 1) and p27kip1 in gastric mucosa carcinogenesis. METHODS: RT-PCR was used to detect TGF beta 1 and p27kip1 mRNAs in normal gastric mucosa, simple hyperplasia, dysplasia and gastric carcinoma tissues respectively. RESULTS: There were expressions of TGF beta 1 and p27kip1 mRNAs in normal gastric mucosa, simple hyperplasia, dysplasia and gastric carcinoma tissues, the expressive levels of TGF beta 1 and p27kip1 mRNAs decreased gradually among the four groups. The expressive level of TGF beta 1 and p27kip1 mRNAs in gastric carcinoma group were significantly lower than those in other three groups(P < 0.05). CONCLUSIONS: The data indicate that TGF beta 1 and p27kip1 may play an important role in the development and carcinogenesis of gastric carcinoma. Semi-quantification PCR technique is effective for detecting mRNA expressive level in tissues. |
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