经皮激光椎间盘减压术对兔椎间盘内磷脂酶A2及神经传导速度的影响

目的探讨经皮激光椎间盘减压术（PLDD）对椎间盘突出症的治疗机制。方法15只大白兔随机分为3组：假手术组、治疗组、非治疗组,治疗组在手术造模的同时进行PLDD治疗。2周后对各实验组动物分别测定L6神经根神经传导速度及椎间盘中的磷脂酶A2（PLA2）活性。结果非治疗组神经传导速度显著低于假手术组（P〈0.001）;治疗组神经传导速度显著快于非治疗组（P〈0.001）。治疗组PLA2活性较非手术组显著降低（P〈0.001）;非治疗组PLA2活性较假手术组显著性升高（P〈0.001）。结论椎间盘纤维环损伤后PLA2水平升高,神经传导速度降低。PLDD可以降低受损后椎间盘内PLA2活性,从而达到治疗的作用。

Effect of Percutaneous Laser Disc Decompression on Phospholipase A2 and Nerve Conduction Velocity in Rabbit with Prolapse of Intervertebral Disc

Objective To explore the mechanism of percutaneous laser disc decompression (PLDD) for treating prolapse of intervertebral disc. Methods 15 rabbits were divided into 3 groups randomly: sham group, model group with PLDD treatment, model group without PLDD treatment. The nerve conduction velocity (NCV) of L6 nerve root and the activity of phospholipase A2 (PLA2) in the intervertebral disc of L5-6 were determined 2 weeks after the initial surgery. Results NCV in the group with PLDD was significantly faster than that in the group without PLDD (P<0.001); NCV in the group without PLDD was significantly lower than that in the sham group (P<0.001). The activity of PLA2 in the group with PLDD was significantly lower than that in the group without PLDD (P<0.001); The activity of PLA2 in the group without PLDD was significantly higher than that in the sham group (P<0.001). Conclusion The activity of PLA2 in the herniated discs is higher than that in normal discs, which result in NCV falls remarkably. The PLDD can reduces chemical factors such as PLA2.